Henry-Toulmé N, Sarthou P, Seman M, Bolard J
Laboratoire de Physique et Chimie Biomoléculaire, CNRS USA 198, Université Paris 6, France.
Mol Cell Biochem. 1989;91(1-2):39-44. doi: 10.1007/BF00228077.
Amphotericin B (AmB) exhibits immunomodulating properties in mice. In vitro studies on lymphocytes, in relation with these properties, are reported here with AmB and two of its derivatives: the N-Fructosyl (N-Fru AmB) and the N-thiopropionyl (AmBSH) derivatives. Interactions of these molecules with thymocytes, a sensitive cell type, demonstrated that the extent of binding is not a toxicity parameter. In contrast, membrane fluidity changes have been observed and appeared to be related to toxicity. Experiments performed with normal B lymphocytes have shown that Amphotericin B derivatives were more potent polyclonal B cell activators than the parent compound. To go further in the understanding of these events, we have investigated in a B cell line WEHI 231, the changes in intracellular Ca2+ and membrane potential induced by AmB and AmBSH. The two polyenes were shown to induce membrane depolarization but no intracellular Ca2+ increase.
两性霉素B（AmB）在小鼠中表现出免疫调节特性。本文报道了关于淋巴细胞的体外研究，涉及这些特性，研究对象为AmB及其两种衍生物：N-果糖基（N-Fru AmB）和N-硫代丙酰基（AmBSH）衍生物。这些分子与敏感细胞类型胸腺细胞的相互作用表明，结合程度不是毒性参数。相反，已观察到膜流动性变化，且似乎与毒性有关。对正常B淋巴细胞进行的实验表明，两性霉素B衍生物比母体化合物更有效地激活多克隆B细胞。为了进一步了解这些事件，我们在B细胞系WEHI 231中研究了AmB和AmBSH诱导的细胞内Ca2+和膜电位变化。这两种多烯被证明可诱导膜去极化，但不会使细胞内Ca2+增加。
