Huang Wen-Ching, Lin Che-Li, Hsu Yi-Ju, Chiu Yen-Shuo, Chen Yi-Ming, Wu Ming-Fang, Huang Chi-Chang, Wang Ming-Fu
Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan City 33301, Taiwan.
Department of Orthopedic Surgery, Shuang Ho Hospital, Taipei Medical University, New Taipei City 23561, Taiwan.
Molecules. 2016 Mar 5;21(3):313. doi: 10.3390/molecules21030313.
The resistant carbohydrates, inulin, and Fibersol-2, belong to soluble dietary fibers and are considered important prebiotics that maintain biological functions, including glucose homeostasis, lipid regulation, colon disease prevention, and prebiotics characteristics. However, few studies have investigated Fibersol-2 alone or in combination with inulin to assess a pooled effect on modulation of hyperlipidemia. We aimed to investigate the effects of this combined supplement (defined as InF) on hamsters fed a 0.2% cholesterol and 10% lard diet (i.e., high-cholesterol diet, HCD) to induce hyperlipidemia. A total of 40 male hamsters were randomly assigned to five groups (n = 8 per group) for treatment: standard diet, vehicle (control); or vehicle or InF supplementation by oral gavage at 0, 864, 1727, or 2591 mg/kg/day for eight weeks, designated HCD, InF-1X, InF-2X, and InF-3X groups, respectively. The hypolipidemic efficacy and safety of InF supplementation was assessed by serum lipid indexes, hepatic and fecal lipid content, and histology. InF supplementation significantly improved serum levels of triacylglycerol (TG) and low-density lipoprotein cholesterol (LDL-C) and the ratio of LDL-C/HDL-C after two-week treatment, and reduced serum total cholesterol (TC) levels after four-week administration. After eight-week supplementation, InF supplementation dose-dependently improved serum levels of TC, TG, HDL-C, and LDL-C; LDL-C/HDL-C ratio; and hepatic TC and TG levels. It inhibited TC absorption by feces elimination. Our study provides experiment-based evidence to support that this prebiotics remedy may be useful in preventing or treating hyperlipidemia.
抗性碳水化合物菊粉和Fibersol-2属于可溶性膳食纤维，被认为是维持生物功能的重要益生元，这些生物功能包括葡萄糖稳态、脂质调节、预防结肠疾病以及具有益生元特性。然而，很少有研究单独研究Fibersol-2或其与菊粉联合使用，以评估对高脂血症调节的综合作用。我们旨在研究这种联合补充剂（定义为InF）对喂食0.2%胆固醇和10%猪油饮食（即高胆固醇饮食，HCD）以诱导高脂血症的仓鼠的影响。总共40只雄性仓鼠被随机分为五组（每组n = 8）进行治疗：标准饮食、赋形剂（对照）；或通过口服灌胃分别以0、864、1727或2591 mg/kg/天的剂量补充赋形剂或InF，持续八周，分别指定为HCD、InF-1X、InF-2X和InF-3X组。通过血清脂质指标、肝脏和粪便脂质含量以及组织学评估InF补充剂的降血脂功效和安全性。治疗两周后，InF补充剂显著改善了血清三酰甘油（TG）和低密度脂蛋白胆固醇（LDL-C）水平以及LDL-C/HDL-C比值，并在给药四周后降低了血清总胆固醇（TC）水平。补充八周后，InF补充剂剂量依赖性地改善了血清TC、TG、HDL-C和LDL-C水平；LDL-C/HDL-C比值；以及肝脏TC和TG水平。它通过粪便排泄抑制了TC吸收。我们的研究提供了基于实验的证据，支持这种益生元疗法可能对预防或治疗高脂血症有用。
