Postgraduate Course on Cellular and Molecular Biology, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
Centre for Scientific and Technological Development, State Foundation on Medical Production and Research, Porto Alegre, Brazil.
J Med Virol. 2016 Oct;88(10):1759-66. doi: 10.1002/jmv.24518. Epub 2016 Mar 22.
A number of studies have demonstrated associations between cytokine gene polymorphisms and outcome of hepatitis B virus (HBV) infection. However, no general consensus has been reached, possibly due to differences between ethnic groups. In this study, 345 individuals living in southern Brazil, including 196 chronic HBV carriers and 149 subjects who had spontaneously recovered from acute infection, were enrolled to evaluate the influence of cytokine gene polymorphisms on the outcome of HBV infection. Most participants were of European descent. Genotyping of IL2-330 G/T, IL4-589C/T, IL6-174 G/C, IL10-592C/A, IL10-1082 A/G, IL17A-197 G/A, IL17A-692 T/C, TNF-α-238 G/A, and TNF-α-308 G/A single nucleotide polymorphisms was performed by using the minisequencing (single base extension) method. By multivariable analysis, a statistically significant association was found between genotypic profile AA + GA in TNF-α-308 and chronic HBV infection (OR, 1.82; 95%CI, 1.01-3.27; P = 0.046). In southern Brazil, the carriers of the -308A allele in the TNF-α gene promoter have a moderately higher risk of becoming chronic carriers in case of HBV infection. In addition, patients with chronic active hepatitis B (n = 60) exhibited a decreased frequency (3.3%) of the TNF-238A allele when compared to that (14.8%) found among asymptomatic HBV carriers (n = 136), suggesting that this could be a protective factor against liver injury (OR, 0.17; 95%CI, 0.04-0.076; P = 0.023). J. Med. Virol. 88:1759-1766, 2016. © 2016 Wiley Periodicals, Inc.
许多研究表明细胞因子基因多态性与乙型肝炎病毒（HBV）感染的结果之间存在关联。然而，由于不同种族之间的差异，尚未达成普遍共识。在这项研究中，招募了居住在巴西南部的 345 个人，包括 196 名慢性 HBV 携带者和 149 名急性感染后自发康复的受试者，以评估细胞因子基因多态性对 HBV 感染结果的影响。大多数参与者是欧洲血统。采用最小测序（单碱基延伸）法对 IL2-330 G/T、IL4-589C/T、IL6-174 G/C、IL10-592C/A、IL10-1082 A/G、IL17A-197 G/A、IL17A-692 T/C、TNF-α-238 G/A 和 TNF-α-308 G/A 单核苷酸多态性进行基因分型。多变量分析发现，TNF-α-308 基因型 AA+GA 与慢性 HBV 感染之间存在统计学显著关联（OR，1.82；95%CI，1.01-3.27；P=0.046）。在巴西南部，TNF-α 基因启动子中 -308A 等位基因的携带者在感染 HBV 时成为慢性携带者的风险略高。此外，与无症状 HBV 携带者（n=136）相比，慢性乙型肝炎患者（n=60）中 TNF-238A 等位基因的频率降低（3.3%比 14.8%），提示这可能是肝脏损伤的保护因素（OR，0.17；95%CI，0.04-0.076；P=0.023）。J. Med. Virol. 88:1759-1766, 2016. © 2016 Wiley Periodicals, Inc.
