臭氧对大鼠腹膜炎模型结肠吻合口的影响。

Effect of ozone on colon anastomoses in rat peritonitis model.

作者信息

Çakır Tuğrul, Aslaner Arif, Tekeli Seçkin Özgür, Avcı Sema, Doğan Uğur, Tekeli Feyza, Soylu Hakan, Akyüz Cebrail, Koç Süleyman, Üstünel İsmail, Yılmaz Necat

机构信息

Department of General Surgery, Antalya Training and Research Hospital, Antalya, Turkey.

Department of Biochemistry, Antalya Training and Research Hospital, Antalya, Turkey.

出版信息

Acta Cir Bras. 2016 Feb;31(2):111-8. doi: 10.1590/S0102-865020160020000005.

DOI:10.1590/S0102-865020160020000005

PMID:26959620

Abstract

PURPOSE

To investigate the effects of medical ozone theraphy on the colon anastomosis of peritonitis model in rats.

METHODS

Eighteen rats were randomly assigned into three equal groups; control, cecal punctuation and colon anastomosis and ozone theraphy. Sepsis was performed with a cecal punctuation in groups 2 and 3. The medical ozone theraphy was administered intraperitonealy for three weeks in group 3 while the other rats received saline injection. At the twenty second day serum were obtained for TNF-α and IL-1β, the colonic burst pressures were measured and colonic tissue samples were obtained for MDA and MPO levels. Histolopatological examination was evaluated with H&E stain, and Ki-67, IL-1β and the VEGF immunostaining densities were also compared.

RESULTS

Intraperitoneal ozone administration reversed TNF-α, IL-1β, MDA and MPO levels and the colonic burst pressures. There was also a significant difference at immunostaining densities of histopathological examination.

CONCLUSION

Medical ozone therapy may contribute to tissue healing by affecting the proliferation and the vascularization thus has benefits on colonic anastomosis at peritonitis in rats.

摘要

目的

探讨医用臭氧疗法对大鼠腹膜炎模型结肠吻合口的影响。

方法

将18只大鼠随机分为三组，每组6只：对照组、盲肠穿刺加结肠吻合组和臭氧治疗组。第2组和第3组通过盲肠穿刺制造脓毒症模型。第3组腹腔内给予医用臭氧疗法，持续三周，而其他大鼠注射生理盐水。在第22天采集血清检测肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β），测量结肠爆破压力，并采集结肠组织样本检测丙二醛（MDA）和髓过氧化物酶（MPO）水平。用苏木精和伊红（H&E）染色进行组织病理学检查，并比较Ki-67、IL-1β和血管内皮生长因子（VEGF）免疫染色密度。