Wang Wei, Li Xiao, Chen Bingzhi, Wang Shuang, Li Chenghuan, Wen Zhiqiang
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
College of Food Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Curr Microbiol. 2016 Jul;73(1):9-14. doi: 10.1007/s00284-016-1020-1. Epub 2016 Mar 10.
The fungus Mycogone perniciosa is a major pathogen of the common button mushroom Agaricus bisporus. Analysis of genetic diversity in M. Perniciosa may assist in developing methods for prophylaxis and treatment of M. Perniciosa infections. For this, it is necessary to classify M. Perniciosa into relevant class groups quickly and efficiently. Random amplified polymorphic DNA (RAPD), inter-simple sequence repeats (ISSR), and sequence-related amplified polymorphism (SRAP) markers were used to obtain genetic fingerprints and assess the genetic variation among 49 strains of M. perniciosa collected from different areas of Fujian Province in China. Analysis of DNA sequence polymorphism revealed two major distinct groups (Group I and Group II). Specific DNA fragments that were identified through RAPD, ISSR, and SRAP markers were sequenced and used for the designing of stable sequence-characterized amplified region (SCAR) markers. The resulting SCAR markers were then validated against the classified groups of M. perniciosa.
有害疣孢霉是双孢蘑菇的一种主要病原菌。分析有害疣孢霉的遗传多样性可能有助于开发预防和治疗有害疣孢霉感染的方法。为此，有必要快速、高效地将有害疣孢霉分类到相关的类别组中。利用随机扩增多态性DNA（RAPD）、简单序列重复区间（ISSR）和序列相关扩增多态性（SRAP）标记来获得遗传指纹，并评估从中国福建省不同地区收集的49株有害疣孢霉菌株之间的遗传变异。DNA序列多态性分析揭示了两个主要的不同组（第一组和第二组）。对通过RAPD、ISSR和SRAP标记鉴定出的特定DNA片段进行测序，并用于设计稳定的序列特征扩增区域（SCAR）标记。然后针对有害疣孢霉的分类组对所得的SCAR标记进行验证。
