Fu Yongping, Wang Xinxin, Li Dan, Liu Yuan, Song Bing, Zhang Chunlan, Wang Qi, Chen Meiyuan, Zhang Zhiwu, Li Yu
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China.
The Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou 350014, China.
Int J Mol Sci. 2016 Sep 22;17(10):1568. doi: 10.3390/ijms17101568.
Outbreaks of wet bubble disease (WBD) caused by Mycogone perniciosa are increasing across the world and seriously affecting the yield of Agaricus bisporus. However, highly WBD-resistant strains are rare. Here, we tested 28 A. bisporus strains for WBD resistance by inoculating M. perniciosa spore suspension on casing soil, and assessed genetic diversity of these strains using 17 new simple sequence repeat (SSR) markers developed in this study. We found that 10 wild strains originating from the Tibetan Plateau in China were highly WBD-resistant strains, and 13 cultivated strains from six countries were highly susceptible strains. A total of 88 alleles were detected in these 28 strains, and the observed number of alleles per locus ranged from 2 to 8. Cluster and genetic structure analysis results revealed the wild resources from China have a relatively high level of genetic diversity and occur at low level of gene flow and introgression with cultivated strains. Moreover, the wild strains from China potentially have the consensus ancestral genotypes different from the cultivated strains and evolved independently. Therefore, the highly WBD-resistant wild strains from China and newly developed SSR markers could be used as novel sources for WBD-resistant breeding and quantitative trait locus (QTL) mapping of WBD-resistant gene of A. bisporus.
由有害疣孢霉引起的湿泡病(WBD)在全球范围内的爆发日益增多,严重影响双孢蘑菇的产量。然而,高抗WBD的菌株十分罕见。在此,我们通过在覆土上接种有害疣孢霉孢子悬浮液,对28个双孢蘑菇菌株进行了WBD抗性测试,并使用本研究开发的17个新的简单序列重复(SSR)标记评估了这些菌株的遗传多样性。我们发现,来自中国青藏高原的10个野生菌株是高抗WBD的菌株,来自6个国家的13个栽培菌株是高感菌株。在这28个菌株中总共检测到88个等位基因,每个位点观察到的等位基因数从2到8不等。聚类和遗传结构分析结果表明,来自中国的野生资源具有较高水平的遗传多样性,且与栽培菌株的基因流动和渗入水平较低。此外,来自中国的野生菌株可能具有与栽培菌株不同的共同祖先基因型,并且是独立进化的。因此,来自中国的高抗WBD野生菌株和新开发的SSR标记可作为双孢蘑菇WBD抗性育种的新资源以及WBD抗性基因的数量性状位点(QTL)定位的新资源。