Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing, 100084, People's Republic of China.
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, People's Republic of China.
Appl Microbiol Biotechnol. 2016 Jul;100(13):6047-53. doi: 10.1007/s00253-016-7435-6. Epub 2016 Mar 10.
Poly(butanediol succinate) (PBS), a biodegradable polymer, was used as both solid carbon source and biofilm carrier for biological nitrate removal process, in which PBS was filled in a packed-bed bioreactor. The denitrification performance and the microbial diversity of biofilm attached on the surface of PBS were investigated. The experimental results showed that the volumetric denitrification rate was 0.60 kg m(-3) day(-1) when NO3-N loading rate was 0.63 kg m(-3) day(-1), and the average NO2-N concentration was below 0.20 mg L(-1). The effluent pH value decreased slightly from a range of 6.98-7.87 to 6.46-7.18. The analysis of microbial community structure of biofilm by pyrosequencing method showed that Proteobacteria was the most abundant phylum (89.87 %), and β-Proteobacteria represented the most abundant class. Among the 76 identified genera, Dechloromonas (10.26 %), Alicycliphilus (9.15 %), Azospira (8.92 %), and Sinobacteraceae-uncultured (8.75 %) were the abundant genera. PBS, as a promising alternative carbon source, is a suitable solid carbon source and biofilm carrier for nitrate removal.
聚丁二酸丁二醇酯（PBS）是一种可生物降解的聚合物，被用作生物硝酸盐去除过程中的固体碳源和生物膜载体，其中 PBS 填充在填充床生物反应器中。研究了附着在 PBS 表面的生物膜的反硝化性能和微生物多样性。实验结果表明，当硝酸盐氮负荷率为 0.63kg/m³·d 时，体积反硝化速率为 0.60kg/m³·d，平均 NO2-N 浓度低于 0.20mg/L。出水 pH 值从 6.98-7.87 略有下降至 6.46-7.18。通过焦磷酸测序法对生物膜微生物群落结构的分析表明，变形菌门（Proteobacteria）是最丰富的门（89.87%），β-变形菌门（β-Proteobacteria）代表最丰富的纲。在 76 个鉴定属中，脱氯菌属（Dechloromonas，10.26%）、环脂酸单胞菌属（Alicycliphilus，9.15%）、固氮螺菌属（Azospira，8.92%）和未培养的辛单胞菌科（Sinobacteraceae-uncultured，8.75%）是丰富的属。PBS 作为一种有前途的替代碳源，是一种适合去除硝酸盐的固体碳源和生物膜载体。
