Ahadian Samad, Zhou Yuanshu, Yamada Shukuyo, Estili Mehdi, Liang Xiaobin, Nakajima Ken, Shiku Hitoshi, Matsue Tomokazu
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario M5S 3G9, Canada.
WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8577, Japan.
Nanoscale. 2016 Apr 7;8(13):7075-84. doi: 10.1039/c5nr07059g.
Graphene was embedded into the structure of mouse embryoid bodies (EBs) using the hanging drop technique. The inclusion of 0.2 mg per mL graphene in the EBs did not affect the viability of the stem cells. However, the graphene decreased the stem cell proliferation, probably by accelerating cell differentiation. The graphene also enhanced the mechanical properties and electrical conductivity of the EBs. Interestingly, the cardiac differentiation of the EB-graphene was significantly greater than that of the EBs at day 5 of culture, as confirmed by high-throughput gene analysis. Electrical stimulation (voltage, 4 V; frequency, 1 Hz; and duration, 10 ms for 2 continuous days) further enhanced the cardiac differentiation of the EBs, as demonstrated by analyses of the cardiac protein and gene expression and the beating activity of the EBs. Taken together, the results demonstrated that graphene played a major role in directing the cardiac differentiation of EBs, which has potential cell therapy and tissue regeneration applications.
使用悬滴法将石墨烯嵌入小鼠胚胎体（EBs）的结构中。在EBs中加入每毫升0.2毫克的石墨烯并不影响干细胞的活力。然而，石墨烯可能通过加速细胞分化降低了干细胞的增殖。石墨烯还增强了EBs的机械性能和导电性。有趣的是，通过高通量基因分析证实，在培养第5天时，含石墨烯的EBs的心脏分化明显大于普通EBs。电刺激（电压4V；频率1Hz；持续时间10ms，连续2天）进一步增强了EBs的心脏分化，这通过对心脏蛋白、基因表达以及EBs的跳动活性分析得到证明。综上所述，结果表明石墨烯在引导EBs的心脏分化中起主要作用，这在细胞治疗和组织再生方面具有潜在应用价值。
