Ahadian Samad, Yamada Shukuyo, Estili Mehdi, Liang Xiaobin, Banan Sadeghian Ramin, Nakajima Ken, Shiku Hitoshi, Matsue Tomokazu, Khademhosseini Ali
WPI-Advanced Institute for Materials Research, Tohoku University, Sendai, 980-8577, Japan.
Graduate School of Environmental Studies, Tohoku University, Sendai, 980-8579, Japan.
Biomed Microdevices. 2017 Sep;19(3):57. doi: 10.1007/s10544-017-0184-1.
We embedded carbon nanotubes (CNTs) in mouse embryoid bodies (EBs) for modulating mechanical and electrical cues of the stem cell niche. The CNTs increased the mechanical integrity and electrical conductivity of the EBs. Measured currents for the unmodified EBs (hereafter, EBs) and the EBs-0.25 mg/mL CNTs were 0.79 and 26.3 mA, respectively, at voltage of 5 V. The EBs had a Young's modulus of 20.9 ± 6.5 kPa, whereas the Young's modulus of the EB-0.1 mg/mL CNTs was 35.2 ± 5.6 kPa. The EB-CNTs also showed lower proliferation and greater differentiation rates compared with the EBs as determined by the expression of pluripotency genes and the analysis of EB sizes. Interestingly, the cardiac differentiation of the EB-CNTs was significantly greater than that of the EBs, as confirmed by high-throughput gene analysis at day 5 of culture. Applying electrical stimulation to the EB-CNTs specifically enhanced the cardiac differentiation and beating activity of the EBs.
我们将碳纳米管（CNTs）嵌入小鼠胚胎体（EBs）中，以调节干细胞微环境的机械和电学信号。碳纳米管提高了胚胎体的机械完整性和导电性。在5V电压下，未修饰的胚胎体（以下简称EBs）和含0.25mg/mL碳纳米管的胚胎体的测量电流分别为0.79mA和26.3mA。胚胎体的杨氏模量为20.9±6.5kPa，而含0.1mg/mL碳纳米管的胚胎体的杨氏模量为35.2±5.6kPa。通过多能性基因表达和胚胎体大小分析确定，与胚胎体相比，含碳纳米管的胚胎体还表现出较低的增殖率和较高的分化率。有趣的是，培养第5天的高通量基因分析证实，含碳纳米管的胚胎体的心脏分化明显大于胚胎体。对含碳纳米管的胚胎体施加电刺激可特异性增强胚胎体的心脏分化和跳动活性。
