Ishida Ryuichi, Sakaguchi Kouta, Matsuzaki Chiaki, Katoh Toshihiko, Ishida Nobuaki, Yamamoto Kenji, Hisa Keiko
Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308, Suematsu, Nonoichi, Ishikawa, 921-8836, Japan.
Nitto Pharmaceutical Industries, Ltd., Kamiueno, Muko, Kyoto, 617-0006, Japan.
Biotechnol Lett. 2016 Apr;38(4):681-7. doi: 10.1007/s10529-015-2024-9. Epub 2016 Mar 9.
A levansucrase from Leuconostoc mesenteroides NTM048 was cloned and expressed and its enzymatic product was characterized.
The fructansucrase gene from Leuconostoc mesenteroides was cloned and expressed in Escherichia coli. The recombinant enzyme was purified as a single protein and its properties investigated. The polymer produced by the recombinant enzyme was identified as levan by various means including TLC and NMRs, and the enzyme was identified as a GH68 levansucrase. The enzyme was optimal at pH 5.5-6 and 30 °C, and its activity was stimulated by Ca(2+). The levan produced by this strain induced IgA production in mice.
Leuconostoc mesenteroides, a probiotic strain, possessed levansucrase which catalyzed the produced levan that had immunomodulating activity.
克隆并表达来自肠系膜明串珠菌NTM048的一种果聚糖蔗糖酶，并对其酶促产物进行表征。
克隆了肠系膜明串珠菌的果聚糖蔗糖酶基因并在大肠杆菌中表达。重组酶被纯化为单一蛋白质并对其性质进行了研究。通过包括薄层色谱法（TLC）和核磁共振（NMR）在内的各种方法鉴定出重组酶产生的聚合物为左聚糖，并且该酶被鉴定为GH68果聚糖蔗糖酶。该酶在pH 5.5 - 6和30℃时最具活性，其活性受Ca(2+)刺激。该菌株产生的左聚糖可诱导小鼠产生IgA。
益生菌菌株肠系膜明串珠菌拥有果聚糖蔗糖酶，该酶催化产生具有免疫调节活性的左聚糖。
