Nikolaev Yu A, Surgucheva N A, Filippova S N
Mikrobiologiia. 2015 Nov-Dec;84(6):673-9.
Regulation of streptomycete growth and development by distant interactions of physical nature was shown using a vial-in-vial experimental setup, providing physical isolation of the inducer and detector cultures. Some effects of distant interaction were observed with Streptomyces netropsis proliferating submerged culture as an inducer and a surface culture of the same strain as a detector. The growth rate of the detector strain doubled. Spore germination was stimulated, as was indicated by a 30% increase in the number of colony-forming units (CRU) in the detector culture plated as a spore suspension. The phase variation spectrum also changed, with an increased share of the colonies of the morphotype predominant under the standard conditions.
通过瓶中瓶实验装置证明了物理性质的远距离相互作用对链霉菌生长和发育的调节作用,该装置实现了诱导菌培养物和检测菌培养物的物理隔离。以诺卡氏链霉菌液体增殖培养物作为诱导菌,同一菌株的固体培养物作为检测菌,观察到了远距离相互作用的一些效应。检测菌株的生长速率翻倍。孢子萌发受到刺激,这表现为以孢子悬液接种的检测菌培养物中菌落形成单位(CFU)数量增加了30%。相变异谱也发生了变化,在标准条件下占主导的形态型菌落比例增加。