绿色产色链霉菌的微周期孢子形成

Microcycle sporulation of Streptomyces viridochromogenes.

作者信息

Koepsel R, Ensign J C

出版信息

Arch Microbiol. 1984 Nov;140(1):9-14. doi: 10.1007/BF00409764.

Abstract

A procedure for submerged culture sporulation of Streptomyces viridochromogenes and S. coelicolor is described. Activated spores were germinated in a complex medium and then shifted to a defined medium containing mineral salts and 2.5 mM L-arginine as carbon and nitrogen source. Sporulation occurred synchronously between 18-24 h incubation. An average of 10 spores were obtained from each original spore in S. viridochromogenes. The microcycle formed spores were nearly identical to surface grown spores with respect to surface morphology, dormancy, germinability and resistance to heat, lysozyme and sonication. The microcycle formed spores differed from surface spores in containing significantly less trehalose, 1.3% compared to 9.7% of the dry weight.

摘要

描述了一种用于绿色产色链霉菌和天蓝色链霉菌深层培养产孢的方法。活化的孢子在复合培养基中萌发，然后转移到含有矿物盐和2.5 mM L-精氨酸作为碳源和氮源的限定培养基中。在培养18 - 24小时之间同步发生产孢。绿色产色链霉菌中每个原始孢子平均获得10个孢子。就表面形态、休眠、萌发能力以及对热、溶菌酶和超声处理的抗性而言，微循环形成的孢子与表面生长的孢子几乎相同。微循环形成的孢子与表面孢子的不同之处在于其海藻糖含量显著较低，干重的1.3% 相比于9.7%。