Guimond-Lischer Stefanie, Ren Qun, Braissant Olivier, Gruner Philipp, Wampfler Bruno, Maniura-Weber Katharina
Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science & Technology, Lerchenfeldstrasse 5, CH-9014 St. Gallen, Switzerland.
Department of Urology, University Hospital Basel, Spitalstrasse 21, CH-4031 Basel, Switzerland and Laboratory for Biomechanics and Biocalorimetry, University of Basel, Klingelbergstrasse 50-70, CH-4056 Basel, Switzerland.
Biointerphases. 2016 Mar 10;11(2):011012. doi: 10.1116/1.4943225.
Fast and efficient osseointegration of implants into bone is of crucial importance for their clinical success; a process that can be enhanced by coating the implant surface with hydroxyapatite (HA) using the vacuum plasma spray technology (VPS). However, bacterial infections, especially the biofilm formation on implant surfaces after a surgery, represent a serious complication. With ever-increasing numbers of antibiotic-resistant bacteria, there is great interest in silver (Ag) as an alternative to classical antibiotics due to its broad activity against Gram-positive and Gram-negative bacterial strains. In the present study, silver ions were introduced into HA spray powder by ion exchange and the HA-Ag powder was applied onto titanium samples by VPS. The Ag-containing surfaces were evaluated for the kinetics of the silver release, its antibacterial effect against Staphylococcus aureus as well as Escherichia coli, and possible cytotoxicity against human bone cells. The HA-Ag coatings with different concentrations of Ag displayed mechanical and compositional properties that fulfill the regulatory requirements. Evaluation of the Ag release kinetic showed a high release rate in the first 24 h followed by a decreasing release rate over the four subsequent days. The HA-Ag coatings showed no cytotoxicity to primary human bone cells while exhibiting antibacterial activity to E. coli and S. aureus.
植入物快速有效地与骨实现骨整合对其临床成功至关重要；这一过程可通过使用真空等离子喷涂技术（VPS）在植入物表面涂覆羟基磷灰石（HA）来增强。然而，细菌感染，尤其是手术后植入物表面形成生物膜，是一种严重的并发症。随着抗生素耐药菌数量不断增加，由于银（Ag）对革兰氏阳性菌和革兰氏阴性菌具有广泛活性，人们对其作为传统抗生素的替代品产生了浓厚兴趣。在本研究中，通过离子交换将银离子引入HA喷涂粉末中，并通过VPS将HA-Ag粉末涂覆在钛样品上。对含银表面进行了银释放动力学、其对金黄色葡萄球菌和大肠杆菌的抗菌效果以及对人骨细胞可能的细胞毒性评估。不同银浓度的HA-Ag涂层显示出符合监管要求的机械和组成特性。银释放动力学评估显示，最初24小时释放速率较高，随后四天释放速率逐渐降低。HA-Ag涂层对原代人骨细胞无细胞毒性，同时对大肠杆菌和金黄色葡萄球菌具有抗菌活性。
