灵敏的检测方法能够检测出健康受试者以及艰难梭菌感染患者血清中针对艰难梭菌毒素A和毒素B的IgG抗体。
Sensitive assays enable detection of serum IgG antibodies against Clostridium difficile toxin A and toxin B in healthy subjects and patients with Clostridium difficile infection.
作者信息
Zhao Xuemei, Bender Florent, Shukla Rajiv, Kang John J, Caro-Aguilar Ivette, Laterza Omar F
机构信息
Translational Molecular Biomarkers, Merck Research Laboratories, Rahway, NJ 07065, USA.
Drug Metabolism & Pharmacokinetics, Novartis Institutes for Biomedical Research, Basel 4057, Switzerland.
出版信息
Bioanalysis. 2016 Apr;8(7):611-23. doi: 10.4155/bio-2015-0033. Epub 2016 Mar 11.
BACKGROUND
Pathogenic Clostridium difficile produces two proinflammatory exotoxins, toxin A and toxin B. Low level of serum antitoxin IgG antibodies is a risk factor for the development of primary and recurrent C. difficile infection (CDI).
RESULTS
We developed and validated two sensitive, titer-based electrochemiluminescence assays for the detection of serum antibody levels against C. difficile toxins A and B. These assays demonstrated excellent precision. The sensitivity of the assays allowed the detection of antitoxin A and antitoxin B IgG antibodies in all tested serum samples during assay validation.
CONCLUSION
The validated titer-based assays enable assessment of antitoxin A and antitoxin B IgG antibodies as potential biomarkers to identify patients with CDI at increased risk for CDI recurrence.
背景
致病性艰难梭菌产生两种促炎性外毒素，即毒素A和毒素B。血清抗毒素IgG抗体水平低是原发性和复发性艰难梭菌感染（CDI）发生的一个危险因素。
结果
我们开发并验证了两种基于滴度的灵敏电化学发光测定法，用于检测血清中针对艰难梭菌毒素A和B的抗体水平。这些测定法显示出极佳的精密度。在测定法验证期间，这些测定法的灵敏度能够检测所有测试血清样本中的抗毒素A和抗毒素B IgG抗体。
结论
经过验证的基于滴度的测定法能够评估抗毒素A和抗毒素B IgG抗体，作为潜在生物标志物以识别有CDI复发高风险的CDI患者。
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