Richter Claudia, Christoph Jan, Lehnart Stephan E, Luther Stefan
Research Group Biomedical Physics, Max Planck Institute for Dynamics and Self-Organization, Am Fassberg 17, 37077, Göttingen, Germany.
DZHK German Center for Cardiovascular Research, Partnersite Göttingen, Göttingen, Germany.
Methods Mol Biol. 2016;1408:293-302. doi: 10.1007/978-1-4939-3512-3_20.
The control of spatiotemporal dynamics in biological systems is a fundamental problem in nonlinear sciences and has important applications in engineering and medicine. Optogenetic tools combined with advanced optical technologies provide unique opportunities to develop and validate novel approaches to control spatiotemporal complexity in neuronal and cardiac systems. Understanding of the mechanisms and instabilities underlying the onset, perpetuation, and control of cardiac arrhythmias will enable the development and translation of novel therapeutic approaches. Here we describe in detail the preparation and optical mapping of transgenic channelrhodopsin-2 (ChR2) mouse hearts, cardiac cell cultures, and the optical setup for photostimulation using digital light processing.
生物系统中时空动态的控制是非线性科学中的一个基本问题，在工程和医学领域有着重要应用。光遗传学工具与先进光学技术相结合，为开发和验证控制神经元和心脏系统时空复杂性的新方法提供了独特机遇。了解心律失常的发生、持续和控制背后的机制及不稳定性，将有助于开发和转化新的治疗方法。在此，我们详细描述转基因2型通道视紫红质（ChR2）小鼠心脏、心脏细胞培养物的制备及光学映射，以及使用数字光处理进行光刺激的光学装置。
