心房心肌细胞的光遗传学工程

Optogenetic Engineering of Atrial Cardiomyocytes.

作者信息

Feola Iolanda, Teplenin Alexander, de Vries Antoine A F, Pijnappels Daniël A

机构信息

Laboratory of Experimental Cardiology, Department of Cardiology, Heart Lung Center Leiden, Leiden University Medical Center, Albinusdreef 2, 2300 RC, Leiden, The Netherlands.

ICIN-Netherlands Heart Institute, Moreelsepark 1, 3511 EP, Utrecht, The Netherlands.

出版信息

Methods Mol Biol. 2016;1408:319-31. doi: 10.1007/978-1-4939-3512-3_22.

DOI:10.1007/978-1-4939-3512-3_22

PMID:26965133

Abstract

Optogenetics is emerging in the cardiology field as a new strategy to explore biological functions through the use of light-sensitive proteins and dedicated light sources. For example, this technology allows modification of the electrophysiological properties of cardiac muscle cells with superb spatiotemporal resolution and quantitative control. In this chapter, the optogenetic modification of atrial cardiomyocytes (aCMCs) from 2-day-old Wistar rats using lentiviral vector (LV) technology and the subsequent activation of the light-sensitive proteins (i.e., ion channels) through light-emitting diodes (LEDs) are described.

摘要

光遗传学作为一种通过使用光敏蛋白和专用光源来探索生物学功能的新策略，正在心脏病学领域崭露头角。例如，这项技术能够以极高的时空分辨率和定量控制来改变心肌细胞的电生理特性。在本章中，将描述使用慢病毒载体（LV）技术对2日龄Wistar大鼠的心房心肌细胞（aCMCs）进行光遗传学修饰，以及随后通过发光二极管（LED）激活光敏蛋白（即离子通道）的过程。