Yang Ping, Wei Jianchang, Li Wanglin, He Feng, Zeng Shanqi, Zhang Tong, Sun Zheng, Cao Jie
Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, Panfu Road No. 1, Guangzhou, 510180, China.
Biotechnol Lett. 2016 Jun;38(6):1043-7. doi: 10.1007/s10529-016-2077-4. Epub 2016 Mar 10.
To explore the roles of growth factor receptor-bound protein 14 (GRB14) in colorectal cancer (CRC) and its correlation with clinicopathological characteristics and prognosis of CRC patients.
GRB14 was localized in the cytoplasm of CRC and benign glandular epithelium cells, showing higher levels in CRC tissues compared with normal colon samples (P < 0.001). High GRB14 was associated with a high pathological grade (P = 0.045), advanced clinical stage (P = 0.018), enhanced tumor invasion (P < 0.001) and lymph node metastasis (P = 0.028). The cancer genome atlas (TCGA) mRNA sequence data showed that GRB14 was upregulated in CRC at an advanced clinical stage (P = 0.011) with enhanced tumor invasion (P < 0.001) and lymph node metastasis (P = 0.014). Kaplan-Meier survival curves revealed that CRC patients with high GRB14 levels had a shorter survival compared with those showing low GRB14 expression (P = 0.007). High GRB14 expression was an independent prognostic factor for CRC patients (HR 2.847, 95 %CI 1.058-7.659; P = 0.038).
GRB14 may be an important cancer promoter that enhances CRC progression. Upregulated GRB14 levels may predict a poor clinical outcome in CRC patients.
探讨生长因子受体结合蛋白14（GRB14）在结直肠癌（CRC）中的作用及其与CRC患者临床病理特征和预后的相关性。
GRB14定位于CRC和良性腺上皮细胞的细胞质中，与正常结肠样本相比，CRC组织中GRB14水平更高（P<0.001）。高GRB14与高病理分级（P=0.045）、临床晚期（P=0.018）、肿瘤侵袭增强（P<0.001）和淋巴结转移（P=0.028）相关。癌症基因组图谱（TCGA）mRNA序列数据显示，在临床晚期的CRC中GRB14上调（P=0.011），肿瘤侵袭增强（P<0.001）和淋巴结转移（P=0.014）。Kaplan-Meier生存曲线显示，GRB14水平高的CRC患者比GRB14表达低的患者生存期短（P=0.007）。高GRB14表达是CRC患者的独立预后因素（HR 2.847，95%CI 1.058-7.659；P=0.038）。
GRB14可能是促进CRC进展的重要癌症促进因子。GRB14水平上调可能预示CRC患者临床预后不良。
