Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), Department Bioengineering, Max-Eyth-Allee 100, D-14469 Potsdam, Germany; Faculty of Agricultural Science, Georg-August-University Göttingen, Büsgenweg 5, D-37077 Göttingen, Germany.
Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), Department Bioengineering, Max-Eyth-Allee 100, D-14469 Potsdam, Germany.
Bioresour Technol. 2016 May;208:200-204. doi: 10.1016/j.biortech.2016.02.104. Epub 2016 Mar 7.
An anaerobic digestion experiment was investigated to evaluate the impact of increasing amounts of ammonium nitrogen due to poultry manure addition on the reactor performance, especially on the microbiome response. The microbial community structure was assessed by using a 16S rRNA gene approach, which was further correlated with the prevalent environmental conditions by using statistical analyses. The addition of 50% poultry manure led to a process disturbance indicated by a high VFA content (almost 10 g(HAc-Eq) L(-1)) in combination with elevated concentrations of ammonium nitrogen (5.9 g NH4(+)-N kg(FM)(-1)) and free ammonia (0.5 g NH3 kg(FM)(-1)). Simultaneously the microbiome, changed from a Bacteroidetes-dominated to a Clostridiales-dominated community accompanied by a shift from the acetoclastic to the hydrogenotrophic pathway. The "new" microbial community was functional redundant as the overall process rates were similar to the former one. A further increase of poultry manure resulted in a complete process failure.
进行了一项厌氧消化实验，以评估由于添加禽粪而导致的铵氮含量增加对反应器性能的影响，特别是对微生物组响应的影响。通过使用 16S rRNA 基因方法评估微生物群落结构，并通过统计分析进一步将其与普遍的环境条件相关联。添加 50%的禽粪导致了过程干扰，表现为高挥发性脂肪酸含量（几乎 10 g(HAc-Eq) L(-1)），同时铵氮（5.9 g NH4(+)-N kg(FM)(-1)）和游离氨（0.5 g NH3 kg(FM)(-1)）浓度升高。同时，微生物组从拟杆菌门为主转变为梭状芽胞杆菌门为主，伴随着从乙酰营养型向氢营养型途径的转变。“新”微生物群落具有功能冗余性，因为整体过程速率与前一种相似。进一步增加禽粪会导致完全的过程失败。
