量子点标记对内皮祖细胞功能和活力的影响。
Effects of Quantum Dot Labeling on Endothelial Progenitor Cell Function and Viability.
作者信息
Molnar Matyas, Friberg Peter, Fu Ying, Brisslert Mikeal, Adams Michael, Chen Yun
机构信息
Department of Molecular and Clinical Medicine/Clinical Physiology, The Sahlgrenska Academy and University Hospital, University of Gothenburg, Gothenburg, Sweden; †Department of Theoretical Chemistry, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden.
Department of Molecular and Clinical Medicine/Clinical Physiology, The Sahlgrenska Academy and University Hospital, University of Gothenburg , Gothenburg , Sweden.
出版信息
Cell Med. 2010 Nov 5;1(2):105-12. doi: 10.3727/215517910X451603. eCollection 2010.
Endothelial progenitor cells (EPC) play an important role in repairing damaged endothelium. An effective imaging method for in vivo tracking of EPCs is essential for understanding EPC-based cell therapy. Fluorescent quantum dots (QDs) have attractive optical characteristics such as extreme brightness and photostability. QDs are currently being investigated as probes for stem cell labeling; however, there is concern about whether QDs can be used safely. We investigated whether quantum dot (QD) labeling would influence EPC viability and function. Rat bone marrow-derived EPCs were cultured and characterized. The cells were labeled with near-infrared-emitting, carboxyl-coated QDs (8 nM) for 24 h. QD labeling efficiency was higher than 97%. Using WST-1 assay, we showed that the viability of the QD-labeled EPCs was not different from that of the control EPCs. Moreover, QD labeling did not influence the ability of EPCs to form capillary tubes on Matrigel and to migrate. The percentage of QD-positive cells decreased with time, probably due to the rapid division of EPCs. These data suggest that the carboxyl-coated QD705 can be useful for labeling EPCs without interrupting their viability and functions.
内皮祖细胞（EPC）在修复受损内皮中发挥着重要作用。一种用于体内追踪EPC的有效成像方法对于理解基于EPC的细胞治疗至关重要。荧光量子点（QD）具有吸引人的光学特性，如极高的亮度和光稳定性。目前正在研究将QD作为干细胞标记的探针；然而，人们担心QD是否可以安全使用。我们研究了量子点（QD）标记是否会影响EPC的活力和功能。培养并鉴定了大鼠骨髓来源的EPC。用发射近红外光的羧基包被的QD（8 nM）标记细胞24小时。QD标记效率高于97%。使用WST-1检测，我们发现QD标记的EPC的活力与对照EPC的活力没有差异。此外，QD标记不影响EPC在基质胶上形成毛细血管管和迁移的能力。QD阳性细胞的百分比随时间下降，可能是由于EPC的快速分裂。这些数据表明，羧基包被的QD705可用于标记EPC，而不会干扰其活力和功能。
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