Li Yong-Hui, Tan Yin-Feng, Cai Hong-Die, Zhang Jun-Qing
Hainan Provincial Key Lab of R&D on Tropic Herbs, Hainan Medical University, No. 3 Xueyuan Road, Haikou, Hainan 571199, PR China; School of Pharmacy, Hainan Medical University, No. 3 Xueyuan Road, Haikou, Hainan 571199, PR China.
Hainan Provincial Key Lab of R&D on Tropic Herbs, Hainan Medical University, No. 3 Xueyuan Road, Haikou, Hainan 571199, PR China; School of Pharmacy, Hainan Medical University, No. 3 Xueyuan Road, Haikou, Hainan 571199, PR China.
J Pharm Biomed Anal. 2016 May 30;124:236-245. doi: 10.1016/j.jpba.2016.02.035. Epub 2016 Feb 27.
Alpinia oxyphylla (Zingiberaceae) is a well-known medicinal plant. Its fruit ("Yi-Zhi-Ren" in Chinese) is used as an anti-diuretic and traditionally used for the treatment of enuresis and reduce urination. Chronic kidney disease (CKD) is a disease with the characteristic of the slowly loss of kidney function and has a prevalence of up to 7-10% in adults. Recent advances in its etiology and pathogenesis are providing more speculative hypotheses focused on integral systems. Using a UPLC-QTOF-MS/MS-based metabolomic platform, we explored the changes of metabolic profiling in plasma/urine simultaneously between chronic kidney disease (CKD) induced from adenine excess and the protective effects of A. oxyphylla extract (AOE). The total twenty-one metabolites (twelve in urine and nine in plasma), up-regulated or down-regulated, were identified and contributed to CKD progress. Among these biomarkers, agmatine, CAMP, 7-methylguanine, hippuric acid, indoxyl sulfate, asparagines, kynurenic acid and p-cresol sulfate were restored back to the control-like level after the treatment of AOE (p<0.05 or 0.01), These findings may be promising to yield a valuable insight into the pathophysiology of CKD and serve as characteristics to explain the mechanisms of AOE.
益智（姜科）是一种著名的药用植物。其果实（中文名为“益智仁”）用作利尿剂，传统上用于治疗遗尿和减少尿量。慢性肾脏病（CKD）是一种以肾功能缓慢丧失为特征的疾病，在成年人中的患病率高达7%-10%。其病因和发病机制的最新进展提供了更多关注整体系统的推测性假说。利用基于超高效液相色谱-四极杆飞行时间串联质谱（UPLC-QTOF-MS/MS）的代谢组学平台，我们同时探究了腺嘌呤过量诱导的慢性肾脏病（CKD）与益智提取物（AOE）的保护作用之间血浆/尿液中代谢谱的变化。共鉴定出21种上调或下调的代谢物（尿液中12种，血浆中9种），它们与CKD的进展有关。在这些生物标志物中，胍丁胺、环磷酸腺苷（CAMP）、7-甲基鸟嘌呤、马尿酸、硫酸吲哚酚、天冬酰胺、犬尿酸和对甲酚硫酸盐在AOE治疗后恢复到类似对照的水平（p<0.05或0.01）。这些发现可能有望为深入了解CKD的病理生理学提供有价值的见解，并作为解释AOE作用机制的特征。
