Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
Henan Engineering Research Center of Clinical Mass Spectrometry for Precision Medicine, Zhengzhou, Henan Province, China.
PLoS One. 2023 Aug 8;18(8):e0285401. doi: 10.1371/journal.pone.0285401. eCollection 2023.
Alpinia oxyphylla Fructus, called Yizhi in Chinese, is the dried fruit of Alpinia oxyphylla Miquel. It has been used in traditional Chinese medicine to treat dementia and memory defects of Alzheimer's disease for many years. However, the underlying mechanism is still unclear. In this study, we used a rat Alzheimer's disease model on intrahippocampal injection of aggregated Aβ1-42 to study the effects of Alpinia oxyphylla Fructus. A brain and plasma dual-channel metabolomics approach combined with multivariate statistical analysis was further performed to determine the effects of Alpinia oxyphylla Fructus on Alzheimer's disease animals. As a result, in the Morris water maze test, Alpinia oxyphylla Fructus had a clear ability to ameliorate the impaired learning and memory of Alzheimer's disease rats. 11 differential biomarkers were detected in AD rats' brains. The compounds mainly included amino acids and phospholipids; after Alpinia oxyphylla Fructus administration, 9 regulated biomarkers were detected compared with the AD model group. In the plasma of AD rats, 29 differential biomarkers, primarily amino acids, phospholipids and fatty acids, were identified; After administration, 23 regulated biomarkers were detected. The metabolic pathways of regulated metabolites suggest that Alpinia oxyphylla Fructus ameliorates memory and learning deficits in AD rats principally by regulating amino acid metabolism, lipids metabolism, and energy metabolism. In conclusion, our results confirm and enhance our current understanding of the therapeutic effects of Alpinia oxyphylla Fructus on Alzheimer's disease. Meanwhile, our work provides new insight into the potential intervention mechanism of Alpinia oxyphylla Fructus for Alzheimer's disease treatment.
益智仁，又称益智，是益智的干燥果实。益智仁在传统中药中已被用于治疗痴呆和阿尔茨海默病的记忆缺陷多年，但作用机制尚不清楚。在这项研究中，我们使用海马内注射聚集的 Aβ1-42 的阿尔茨海默病大鼠模型来研究益智仁的作用。进一步采用脑和血浆双通道代谢组学方法结合多变量统计分析来确定益智仁对阿尔茨海默病动物的影响。结果表明，在 Morris 水迷宫测试中，益智仁能明显改善阿尔茨海默病大鼠的学习和记忆障碍。在 AD 大鼠的大脑中检测到 11 个差异生物标志物，这些化合物主要包括氨基酸和磷脂；与 AD 模型组相比，益智仁给药后检测到 9 个调节生物标志物。在 AD 大鼠的血浆中，鉴定出 29 个差异生物标志物，主要为氨基酸、磷脂和脂肪酸；给药后检测到 23 个调节生物标志物。调节代谢物的代谢途径表明，益智仁主要通过调节氨基酸代谢、脂质代谢和能量代谢来改善 AD 大鼠的记忆和学习能力缺陷。总之，我们的研究结果证实并加深了我们对益智仁治疗阿尔茨海默病疗效的认识。同时，我们的工作为益智仁治疗阿尔茨海默病的潜在干预机制提供了新的见解。
