Yi C G, Xia W, Zhang L X, Zhen Y, Shu M G, Han Y, Guo S Z
Department of Plastic Surgery, Xijing Hospital, The Fourth Military Medical University, #15 Changlexilu, Xi'an City, Shaanxi Province 710032, China.
J Plast Reconstr Aesthet Surg. 2007;60(3):272-8. doi: 10.1016/j.bjps.2006.01.052. Epub 2006 Jun 15.
The objective of this study was to determine the effects of adenovirus-mediated vascular endothelial growth factor (Ad-VEGF) on the angiogenesis and survival of free-fat tissue transplantation in nude mice. Thirty 6-week-old CD-1 nude male mice were injected with 1ml fat tissue (harvested by suction-assisted lipectomy from the breast of humans) in the subcutaneous of scalp and were randomised into three groups of 10 animals each. Group 1 was the study group, in which Ad-VEGF was mixed with transplanted fat tissue and injected into mice. In group 2, adenovirus-mediated green fluorescent protein (Ad-GFP) gene was mixed with transplanted fat tissue and injected into the mice. In group 3, normal saline alone was used. Both group 2 and group 3 are control groups. The animals were euthanised 15 weeks after the procedure. The fat survival weight and volume of the study group were significantly greater than those of two control groups (p<0.05). Light microscopical examination of haematoxylin and eosin-stained slides of the dissected fat 15 weeks after injection was performed in group 1 and group 2. Less cyst formation and fibrosis, indicating improved quality of the injected fat, can be obtained by the addition of Ad-VEGF. Vascular density was evaluated at the microvascular level through the use of light microscopic sections of the central part of the fat tissue at 15 weeks after injection by von Willebrand factor staining. Histological evaluation showed that capillary density increased markedly in the study group mice. Mice of the study group disclosed significantly higher VEGF protein levels detected by ELISA assay of plasma samples obtained from the mice after the fat injection (day 1, 4, 7 and 28; p<0.01) at each time point than the mice of the two control groups. The findings reported in this study indicate that the VEGF gene therapy can enhance the survival and the quality of grafted fat tissue, which may be due to induction of angiogenesis.
本研究的目的是确定腺病毒介导的血管内皮生长因子（Ad-VEGF）对裸鼠游离脂肪组织移植血管生成及存活的影响。30只6周龄的CD-1雄性裸鼠在头皮下注射1ml脂肪组织（通过吸脂术从人类乳房获取），并随机分为三组，每组10只动物。第1组为研究组，将Ad-VEGF与移植脂肪组织混合后注射到小鼠体内。第2组将腺病毒介导的绿色荧光蛋白（Ad-GFP）基因与移植脂肪组织混合后注射到小鼠体内。第3组仅使用生理盐水。第2组和第3组均为对照组。术后15周对动物实施安乐死。研究组的脂肪存活重量和体积显著大于两个对照组（p<0.05）。对第1组和第2组注射后15周解剖的脂肪苏木精-伊红染色玻片进行光学显微镜检查。添加Ad-VEGF可减少囊肿形成和纤维化，表明注射脂肪的质量得到改善。通过对注射后15周脂肪组织中央部分的光学显微镜切片进行von Willebrand因子染色，在微血管水平评估血管密度。组织学评估显示研究组小鼠的毛细血管密度显著增加。研究组小鼠脂肪注射后（第1、4、7和28天；p<0.01）每次时间点采集的血浆样本经ELISA检测，发现其VEGF蛋白水平显著高于两个对照组小鼠。本研究报告的结果表明，VEGF基因治疗可提高移植脂肪组织的存活率和质量，这可能是由于诱导血管生成所致。
