Xi'an, Shaanxi, People's Republic of China From the Institute of Plastic Surgery, Xijing Hospital, and the Departments of Oral and Maxillofacial Surgery and Implantation, School of Stomatology, Fourth Military Medical University.
Plast Reconstr Surg. 2012 Sep;130(3):398e-406e. doi: 10.1097/PRS.0b013e31825dbfd3.
Autologous fat transplantation has become a prevalent option for soft-tissue augmentation throughout the body. However, there is still much controversy over whether the fat grafts have survived or have been replaced in the recipient sites and over how the vessels grow.
After C57BL/6-gfp mice and C57BL/6 mice were paired randomly, the inguinal fat was excised and cut into pieces with scissors, and the adipose granules, approximately 0.2 ml (0.195 g), were transplanted subcutaneously with syringes to the dorsa of the paired mice. Samples were obtained at different time intervals: 3 days, 7 days, 2 weeks, 4 weeks, 2 months, 3 months, and 4 months after transplantation. Each fat sample was weighed to evaluate the graft volume. Histology, origin, and densities of neovascularization were examined by immunohistochemical staining.
At 4 months, there was no significant difference in either graft survival or histologic evaluation. Histologic evaluation manifested the normal physiologic process of inflammation, neovascularization, remodeling, and maturity at different time intervals. At the endpoint, the immunohistochemical staining of CD34 showed that the difference in capillary density of the fat graft-31.3 ± 3.9 capillaries/mm on the dorsa of the C57BL/6-gfp mice and 29.6 ± 3.2 capillaries/mm on the dorsa of the C57BL/6 mice-was not statistically significant. The α-smooth muscle actin staining indicated that there were neovascularized vessels in both C57BL/6-gfp and C57BL/6 fat grafts.
Fat grafts can survive and neovascularized vessels can grow from the recipient sites. Fat transplantation is feasible and will be applied more widely if fat graft survival is improved.
自体脂肪移植已成为全身软组织填充的一种流行选择。然而，关于脂肪移植物是否在受区存活或被替代，以及血管如何生长，仍存在很多争议。
将 C57BL/6-gfp 小鼠和 C57BL/6 小鼠随机配对，切除腹股沟脂肪，用剪刀剪成小块，用注射器将约 0.2ml（0.195g）脂肪颗粒移植到配对小鼠的背部皮下。在不同的时间间隔（移植后 3 天、7 天、2 周、4 周、2 个月、3 个月和 4 个月）获取样本。每个脂肪样本称重以评估移植物体积。通过免疫组织化学染色检查新生血管的起源和密度。
在 4 个月时，移植物存活率或组织学评估均无显著差异。组织学评估显示在不同的时间间隔，有炎症、新生血管形成、重塑和成熟的正常生理过程。在终点时，CD34 的免疫组织化学染色显示，C57BL/6-gfp 小鼠背部脂肪移植物的毛细血管密度（31.3±3.9 个毛细血管/mm）与 C57BL/6 小鼠背部的毛细血管密度（29.6±3.2 个毛细血管/mm）之间的差异无统计学意义。α-平滑肌肌动蛋白染色表明，C57BL/6-gfp 和 C57BL/6 脂肪移植物中均有新生血管化的血管。
脂肪移植物可以存活，受区可以生长新生血管。如果脂肪移植物的存活率提高，脂肪移植将更可行，并得到更广泛的应用。
