Centre for Research, Department of Biotechnology, Kamaraj College of Engineering and Technology, Virudhunagar, Tamil Nadu, India.
Department of Chemical Engineering, Indian Institute of Technology - BHU, Varanasi, India.
Bioresour Technol. 2016 Aug;213:39-43. doi: 10.1016/j.biortech.2016.02.126. Epub 2016 Mar 3.
The degradation of Reactive Red 120 using Bacillus cohnii RAPT1 immobilized on polyurethane was studied. Initial experiments indicated that the percentage removal of dye in immobilized batch was significantly higher than batch (without immobilization). The optimum process parameters such as effect of dye concentration, time of immobilization on Poly Urethane Foam, initial inoculum size, pH and temperature for removal of dye were investigated and was found as 200ppm, 36h, 300*10(6) colony forming units/ml, 8.0 and 35°C respectively. Under optimum conditions, 100% removal of dye was obtained within 4h. The kinetics of biodegradation for the batch with free cells and immobilised packed batch was found to be IInd order with kinetic constant and initial rate of reaction as 0.0408, 0.084L/(mgday) and 1632, 3360 (mg/Lday) respectively.
使用固定在聚氨酯上的凝结芽胞杆菌 RAPT1 降解反应红 120 进行了研究。初步实验表明，固定批量处理中染料的去除率明显高于批量处理（无固定化）。研究了去除染料的最佳工艺参数，如染料浓度、固定在聚氨酯泡沫上的时间、初始接种量、pH 值和温度等，结果分别为 200ppm、36h、300×10(6)个菌落形成单位/ml、8.0 和 35°C。在最佳条件下，染料在 4 小时内被完全去除。发现游离细胞批次和固定化填充批次的生物降解动力学为二级，动力学常数和初始反应速率分别为 0.0408、0.084L/（mg·天）和 1632、3360（mg/L·天）。
