Shentu Xu-Ping, Li Dan-Ting, Xu Jian-Feng, She Liang, Yu Xiao-Ping
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, PR China.
Arkansas Biosciences Institute and College of Agriculture, Arkansas State University, Jonesboro, AR, USA.
Pestic Biochem Physiol. 2016 Mar;128:16-21. doi: 10.1016/j.pestbp.2015.10.010. Epub 2015 Oct 16.
Yeast-like symbiotes (YLS) are endosymbionts that are closely related to the growth, development and reproduction of their host, the brown planthopper (BPH), Nilaparvata lugens Stål (Hemiptera: Delphacidae). In order to understand the relationship between the population of YLS in BPH cells and the survival rate of BPH, eight different fungicides were applied to rice plants infested by BPH, and the number of YLS and mortality of BPH were determined. Three of the fungicides, 27% toyocamycin & tetramycin P & tetrin B & tetramycin A, 0.01% trichodermin, and 75% trifloxystrobin & tebuconazole WG, were found to significantly reduce the number of YLS in BPH, subsequently causing a high mortality of BPH. The three fungicides were each mixed with a commonly used insecticide-imidacloprid, and the fungicide/insecticide mixtures could cause a marked reduction in YLS number in BPH, resulting in a significantly higher mortality of BPH than did the imidacloprid alone. The mixture of 27% toyocamycin & tetramycin P & tetrin B & tetramycin A with imidacloprid showed the best inhibitory effect on BPH population. Our study demonstrated a high dependence of the BPH survival rate on the number of YLS harbored in BPH fat-body cells. It implies that using specific fungicides as an additive to imidacloprid for controlling BPH could be a novel way to enhance the efficacy of insecticide, minimizing the use of imidacloprid in paddy fields.
类酵母共生体（YLS）是一种内共生体，与它们的宿主褐飞虱（BPH），即Nilaparvata lugens Stål（半翅目：飞虱科）的生长、发育和繁殖密切相关。为了了解褐飞虱细胞中类酵母共生体的数量与褐飞虱存活率之间的关系，将八种不同的杀菌剂施用于受褐飞虱侵害的水稻植株上，并测定了类酵母共生体的数量和褐飞虱的死亡率。发现其中三种杀菌剂，即27% 春雷霉素 & 四霉素P & 四霉素B & 四霉素A、0.01% 木霉菌素和75% 肟菌酯 & 戊唑醇水分散粒剂，能显著减少褐飞虱体内类酵母共生体的数量，随后导致褐飞虱的高死亡率。这三种杀菌剂分别与一种常用杀虫剂吡虫啉混合，杀菌剂/杀虫剂混合物可使褐飞虱体内类酵母共生体数量显著减少，导致褐飞虱死亡率显著高于单独使用吡虫啉。27% 春雷霉素 & 四霉素P & 四霉素B & 四霉素A与吡虫啉的混合物对褐飞虱种群的抑制效果最佳。我们的研究表明，褐飞虱的存活率高度依赖于其脂肪体细胞中类酵母共生体的数量。这意味着使用特定的杀菌剂作为吡虫啉的添加剂来控制褐飞虱可能是一种提高杀虫剂功效的新方法，可最大限度地减少稻田中吡虫啉的使用。
