Luo F, Jia R, Ying S, Wang Z, Wang F
Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing, 210095, China.
Jiangsu Engineering Technology Research Center of Meat Sheep & Goat Industry, Nanjing Agricultural University, Nanjing, 210095, PR China.
Anim Genet. 2016 Jun;47(3):354-64. doi: 10.1111/age.12427. Epub 2016 Mar 11.
Nutrition is an important factor that regulates reproductive performance of sheep and affects follicle development. However, the correlation between nutrition and follicle development is poorly understood at the molecular level. To study its possible molecular mechanisms, we performed expression profiling of granulosa cells isolated from sheep that were fed different levels of nutrition levels during the luteal phase. To do this, ewes received a maintenance diet (M), and their estrus was synchronized by intravaginal progestogen sponges for 12 days. Ewes were randomly divided into the short-term dietary-restricted group (R; 0.5 × M) and the nutrient-supplemented group (S; 1.5 × M). RNA samples were extracted from granulosa cells. Transcriptome libraries from each group were constructed by Illumina sequencing. Among 18 468 detected genes, 170 genes were significantly differentially expressed, of which 140 genes were upregulated and 30 genes were downregulated in group S relative to group R. These genes could be candidates regulating follicular development in sheep. Gene Ontology, KEGG and clustering analyses were performed. Genes related to oocyte meiosis, such as ADCY7, were upregulated. We identified two important groups of related genes that were upregulated with improved nutrition: one group comprising the genes PTGS2, UCP2 and steroidogenic acute regulatory protein and the other group comprising interleukin-1A and interleukin-1B. The genes within each group showed similar expression patterns. Additionally, all five genes are involved in the reproduction process. Quantitative real-time PCR was performed to validate the results of expression profiling. These data in our study are an abundant genomic resource to expand the understanding of the molecular and cellular events underlying follicle development.
营养是调节绵羊繁殖性能并影响卵泡发育的重要因素。然而，在分子水平上，人们对营养与卵泡发育之间的相关性了解甚少。为了研究其可能的分子机制，我们对在黄体期饲喂不同营养水平的绵羊分离出的颗粒细胞进行了表达谱分析。为此，母羊接受维持日粮（M），并通过阴道内孕酮海绵同步发情12天。母羊被随机分为短期饮食限制组（R；0.5×M）和营养补充组（S；1.5×M）。从颗粒细胞中提取RNA样本。通过Illumina测序构建每组的转录组文库。在检测到的18468个基因中，有170个基因显著差异表达，其中相对于R组，S组中有140个基因上调，30个基因下调。这些基因可能是调节绵羊卵泡发育的候选基因。进行了基因本体论、KEGG和聚类分析。与卵母细胞减数分裂相关的基因，如ADCY7，被上调。我们确定了两组随着营养改善而上调的重要相关基因：一组包括PTGS2、UCP2和类固醇生成急性调节蛋白基因，另一组包括白细胞介素-1A和白细胞介素-1B。每组中的基因显示出相似的表达模式。此外，所有五个基因都参与生殖过程。进行了定量实时PCR以验证表达谱分析的结果。我们研究中的这些数据是丰富的基因组资源，有助于扩大对卵泡发育潜在分子和细胞事件的理解。
