División de Estudios de Posgrado e Investigación, TecNM/Instituto Tecnológico de Conkal, Av. Tecnológico S/N, Conkal, Yucatán 97345, Mexico.
Departamento de Recursos del Mar, Cinvestav Unidad Mérida, Mérida, Yucatán 97310, Mexico.
Genes (Basel). 2019 May 10;10(5):358. doi: 10.3390/genes10050358.
The Pelibuey sheep () is an indigenous breed distributed in the tropical regions of Mexico. The prolificacy of this sheep is on average from 1 to 1.5 lambs, being an important breeding characteristic that owners seek to increase with the purpose of economic improvements. New-generation RNA sequencing technology has been used to identify the genes that are expressed in the ovarian tissue of sheep that have two or more lambs per parturition, as well as to elucidate the metabolic pathways that are affected by the expression of these genes, with the purpose of better understanding the prolificacy in the sheep. In the present study, the transcriptional expression of multiparous and uniparous sheep was compared using RNA sequencing. Multiparous (M group) and uniparous (U group) sheep that had a genealogical record for three generations (M, n = 5 and U, n = 5) were selected. RNA was extracted from ovarian tissue and subsequently used to prepare the libraries that were sequenced using the Illumina NextSeq500 platform. A total of 31,575 genes were detected from the transcriptomic analysis of which 4908 were significantly expressed (-value ≤ 0.001) in the ovary of sheep. Subsequently, a second filter was carried out to evaluate the false discovery rate (FDR) and select those genes with -values ≤ 0.05 and values of expression ≥ 1 (log2), obtaining 354 differential expressed genes (DEG): 120 genes up-regulated and 234 genes down-regulated in the group M with respect to the group U. Through Gene Ontology (GO) and metabolic analysis, we obtained information on the function of differentially expressed genes, and its importance in the reproduction of multiparous sheep. This result suggest that genes identified in the present study participate in the development of the final stages of follicles.
佩里贝尤羊（Pelibuey sheep）是一种分布在墨西哥热带地区的本土品种。该羊的繁殖力平均为每胎产 1 到 1.5 只羔羊，是所有者寻求增加的重要繁殖特征，目的是提高经济效益。新一代 RNA 测序技术已被用于鉴定在每胎产仔数为 2 只或更多的绵羊卵巢组织中表达的基因，并阐明受这些基因表达影响的代谢途径，以更好地了解绵羊的繁殖力。在本研究中，使用 RNA 测序比较了多胎和单胎绵羊的转录表达。选择了有三代谱系记录的多胎（M 组）和单胎（U 组）绵羊（M，n = 5 和 U，n = 5）。从卵巢组织中提取 RNA，随后用于制备文库，使用 Illumina NextSeq500 平台对文库进行测序。从转录组分析中检测到 31575 个基因，其中 4908 个基因在绵羊卵巢中显著表达（-值≤0.001）。随后，进行了第二次筛选，以评估错误发现率（FDR）并选择那些-值≤0.05 且表达值≥1（log2）的基因，得到 354 个差异表达基因（DEG）：M 组相对于 U 组有 120 个基因上调和 234 个基因下调。通过基因本体论（GO）和代谢分析，我们获得了差异表达基因功能的信息及其在多胎绵羊繁殖中的重要性。这一结果表明，本研究中鉴定的基因参与了卵泡最后阶段的发育。
