Engineering Research Center of Pharmaceutical Process Chemistry, East China University of Science and Technology, Shanghai, 200237, China.
Engineering Research Center of Pharmaceutical Process Chemistry, East China University of Science and Technology, Shanghai, 200237, China.
Talanta. 2020 Mar 1;209:120525. doi: 10.1016/j.talanta.2019.120525. Epub 2019 Oct 31.
A novel polar stationary phase for hydrophilic interaction chromatography (HILIC) is prepared by surface-initiated polymerization of hyperbranched polyglycerol onto silica. Such polymerization offers an easy way to tune the cabon load. The stationary phase showed obvious hydrogen bonding interaction and typical HILIC character, exhibiting nealy-universal separation ability towards polar analytes. Good peak shape and high separation efficiency was observed for model polar analytes, including nucleosides and nucleic bases, aromatic acids, organic bases, vitamins, saccharides, amino acids, e.g. 35,000-58,000 plate counts per meter for five water-soluble vitamins.
一种新型的亲水性相互作用色谱（HILIC）的极性固定相，是通过超支化聚甘油在硅胶表面引发聚合制备的。这种聚合提供了一种简单的方法来调整碳载量。固定相表现出明显的氢键相互作用和典型的 HILIC 特性，对极性分析物具有几乎通用的分离能力。对于模型极性分析物，如核苷和碱基、芳香酸、有机碱、维生素、糖、氨基酸等，观察到良好的峰形和高分离效率，5 种水溶性维生素的理论塔板数达到 35000-58000/m。
