Shi Huimin, Cui Yi, Gong Yijun, Feng Suling
School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China; Department of Basic Medical Sciences, Zhengzhou Shuqing Medical College, Zhengzhou 450064, China.
School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2016 May 15;161:150-4. doi: 10.1016/j.saa.2016.02.023. Epub 2016 Feb 27.
A fluorescent probe has been developed for the determination of guanine based on the quenched fluorescence signal of Cu(2+)/eosin Y. Cu(2+) interacted with eosin Y, resulting in fluorescence quenching. Subsequently, with the addition of guanine to the Cu(2+)/eosin Y system, guanine reacted with Cu(2+) to form 1:1 chelate cation, which further combined with eosin Y to form a 1:1 ternary ion-association complex by electrostatic attraction and hydrophobic interaction, resulting in significant decrease of the fluorescence. Hence, a fluorescent system was constructed for rapid, sensitive and selective detection of guanine with a detection limit as low as 1.5 nmol L(-1) and a linear range of 3.3-116 nmol L(-1). The method has been applied satisfactorily to the determination of guanine in DNA and urine samples with the recoveries from 98.7% to 105%. This study significantly expands the realm of application of ternary ion-association complex in fluorescence probe.
基于Cu(2+)/曙红Y的荧光信号猝灭，开发了一种用于测定鸟嘌呤的荧光探针。Cu(2+)与曙红Y相互作用，导致荧光猝灭。随后，向Cu(2+)/曙红Y体系中加入鸟嘌呤，鸟嘌呤与Cu(2+)反应形成1:1螯合阳离子，该阳离子通过静电吸引和疏水相互作用进一步与曙红Y结合形成1:1三元离子缔合物，导致荧光显著降低。因此，构建了一种荧光体系，用于快速、灵敏和选择性地检测鸟嘌呤，检测限低至1.5 nmol L(-1)，线性范围为3.3-116 nmol L(-1)。该方法已成功应用于DNA和尿液样品中鸟嘌呤的测定，回收率在98.7%至105%之间。本研究显著扩展了三元离子缔合物在荧光探针中的应用领域。
