Guangdong Provincial Engineering Research Center of Public Health Detection and Assessment, Guangdong Pharmaceutical University, Guangzhou, 510310, Guangdong, China.
Guangdong Engineering Research Center of Natural Products and New Drugs, Guangdong Provincial University Engineering Technology Research Center of Natural Products and Drugs, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong, China.
Anal Bioanal Chem. 2021 Feb;413(5):1429-1440. doi: 10.1007/s00216-020-03107-4. Epub 2021 Jan 5.
The sensitive chitosan (CTS) detection methods based on the resonance Rayleigh scattering (RRS) quenching method and fluorescence quenching of Eosin Y were put forward. In the HAC-NaAC buffer solution, Eosin Y interacted with Triton X-100 to generate the binary complex which served as the RRS spectral probe. When CTS was interacted with the binary complex, the RRS intensity decreased with the increase of CTS. At the same time, the fluorescence intensity of Eosin Y decreased in the presence of Triton X-100, and the fluorescence intensity of "Eosin Y+Triton X-100" system further decreased when CTS was added. So it was further proved that there was a forming complex in "Eosin Y+Triton X100+CTS" system. The interaction was characterized by zeta potential, RRS, fluorescence spectrum, and UV-Vis spectroscopy. Under optimal conditions, there was a good linear relationship between the RRS decreased intensity (ΔI) and the concentration of CTS in the range of 0.05-1.30 μg/mL, with a regression equation of ΔI = 1325c + 73.66 and correlation coefficient (R) of 0.9907. The detection limit was 0.0777 μg/mL. Likewise, the linear range of the fluorescence quenching was 0.03-1.30 μg/mL; the regression equation was ΔF = 1926c + 294.0 with R = 0.9800 under fluorescence quenching. The detection limit was 0.0601 μg/mL. Therefore, the dual-channel sensor for the determination of CTS was applied to the health products, and the results were satisfactory. The t test result showed that there was no statistical difference between the two methods.
提出了基于共振瑞利散射（RRS）猝灭法和曙红 Y 荧光猝灭的灵敏壳聚糖（CTS）检测方法。在 HAC-NaAC 缓冲溶液中，曙红 Y 与 Triton X-100 相互作用生成二元配合物，用作 RRS 光谱探针。当 CTS 与二元配合物相互作用时，RRS 强度随 CTS 的增加而降低。同时，在存在 Triton X-100 的情况下，曙红 Y 的荧光强度降低，当加入 CTS 时，“曙红 Y+Triton X-100”体系的荧光强度进一步降低。因此，进一步证明了“曙红 Y+Triton X100+CTS”体系中存在形成的配合物。通过 zeta 电位、RRS、荧光光谱和紫外可见光谱对相互作用进行了表征。在最佳条件下，在 0.05-1.30 μg/mL 范围内，RRS 降低强度（ΔI）与 CTS 浓度之间存在良好的线性关系，回归方程为ΔI=1325c+73.66，相关系数（R）为 0.9907。检测限为 0.0777μg/mL。同样，荧光猝灭的线性范围为 0.03-1.30μg/mL；荧光猝灭下的回归方程为ΔF=1926c+294.0，R=0.9800。检测限为 0.0601μg/mL。因此，将双通道传感器用于测定 CTS 的测定，并将结果应用于保健品中，结果令人满意。t 检验结果表明两种方法之间无统计学差异。
