Mikula I, Rosocha J, Pilipcinec E
Acta Vet Hung. 1989;37(3):219-26.
Eight calves were immunized with live auxotrophic Salmonella typhimurium mutants (aro -SL 1479, gal E 3821) and twelve calves with phenol-killed whole-cell S. typhimurium vaccine, respectively. The clinical status of the animals was followed and serial reisolation of vaccine and challenge strains from faeces was attempted. The immunization of calves with the live aro- auxotrophic S. typhimurium SL 1479 mutant proved to be unsuitable due to the death of calves after revaccination. The calves immunized with live auxotrophic gal E S. typhimurium CCM 3821 mutant proved to be protected against challenge with virulent S. typhimurium 4/5 strain administered orally at a dose of 10(6) colony forming units (CFU). The postvaccination complications showed serious shortcomings. The immunization of calves with three doses of whole-cell inactivated vaccine containing 5 strains of S. typhimurium was effective against oral challenge with virulent S. typhimurium 4/5 at a dose of 10(6) CFU.
分别用活的营养缺陷型鼠伤寒沙门氏菌突变株（aro -SL 1479、gal E 3821）对8头小牛进行免疫，用苯酚灭活的鼠伤寒沙门氏菌全细胞疫苗对12头小牛进行免疫。跟踪动物的临床状况，并尝试从粪便中连续重新分离疫苗株和攻击株。用活的aro营养缺陷型鼠伤寒沙门氏菌SL 1479突变株对小牛进行免疫被证明不合适，因为再次接种疫苗后小牛死亡。用活的营养缺陷型gal E鼠伤寒沙门氏菌CCM 3821突变株免疫的小牛被证明能够抵御口服剂量为10(6) 菌落形成单位（CFU）的强毒株鼠伤寒沙门氏菌4/5的攻击。疫苗接种后的并发症显示出严重的缺陷。用含有5株鼠伤寒沙门氏菌的三剂全细胞灭活疫苗对小牛进行免疫，可有效抵御口服剂量为10(6) CFU的强毒株鼠伤寒沙门氏菌4/5的攻击。
