Habasha F G, Smith B P, Schwartz L, Ardans A, Reina-Guerra M
Am J Vet Res. 1985 Jul;46(7):1415-21.
Migration of bovine macrophages under agarose was used to assess cellular immunity in 7 nonvaccinated calves and 9 calves vaccinated with Salmonella typhimurium. The 9 vaccinated calves were allotted to 4 groups. Group I calves were vaccinated twice orally with small doses of virulent S typhimurium; group II calves were vaccinated twice orally with genetically altered aromatic-dependent (aro-) S typhimurium SL3261; group III calves were vaccinated twice IM with small doses of virulent S typhimurium; and group IV calves were vaccinated twice IM with aro- S typhimurium SL1479. Samples of blood were obtained from these calves at 2 weeks after the 2nd vaccinal dose was given, and lymphocytes were harvested, using lymphocyte separation medium. Lymphocytes in serum-free medium were then incubated with S typhimurim antigen for 48 hours. Lymphocytes were then transferred to antigen-free medium and incubated for 48 hours, and the supernatant was assayed for the migration-inhibition factor (MIF). Lymphocyte supernatant was assayed for MIF by incubating it for 48 hours with 2.0 X 10(4) alveolar macrophages in agar wells. The macrophage migration distance was measured and compared with control values. Macrophage migration was inhibited in the presence of supernatant of lymphocytes from vaccinated calves that had been incubated with antigen, indicating the presence of the MIF in the supernatant. Migration distances, as a percentage of control, were 33% for group I calves (oral vaccination, virulent vaccinal organism), 60% for group II calves (oral vaccination, aro- vaccinal organism), 41% for group III (IM vaccination, virulent organism), and 25% for group IV (IM vaccination, aro- vaccinal organism).(ABSTRACT TRUNCATED AT 250 WORDS)
利用琼脂糖下牛巨噬细胞的迁移来评估7头未接种疫苗的小牛和9头接种鼠伤寒沙门氏菌疫苗的小牛的细胞免疫。9头接种疫苗的小牛被分为4组。第一组小牛口服小剂量有毒力的鼠伤寒沙门氏菌进行两次疫苗接种；第二组小牛口服基因改造的芳香族依赖性（aro-）鼠伤寒沙门氏菌SL3261进行两次疫苗接种；第三组小牛肌肉注射小剂量有毒力的鼠伤寒沙门氏菌进行两次疫苗接种；第四组小牛肌肉注射aro-鼠伤寒沙门氏菌SL1479进行两次疫苗接种。在第二次接种疫苗剂量后2周从这些小牛采集血样，使用淋巴细胞分离培养基收获淋巴细胞。然后将无血清培养基中的淋巴细胞与鼠伤寒沙门氏菌抗原孵育48小时。接着将淋巴细胞转移到无抗原培养基中孵育48小时，并检测上清液中的迁移抑制因子（MIF）。通过将淋巴细胞上清液与琼脂孔中2.0×10⁴个肺泡巨噬细胞孵育48小时来检测MIF。测量巨噬细胞迁移距离并与对照值进行比较。在与抗原孵育过的接种疫苗小牛的淋巴细胞上清液存在的情况下，巨噬细胞迁移受到抑制，表明上清液中存在MIF。作为对照百分比的迁移距离，第一组小牛（口服接种，有毒力的疫苗菌株）为33%，第二组小牛（口服接种，aro-疫苗菌株）为60%，第三组（肌肉注射接种，有毒力的菌株）为41%，第四组（肌肉注射接种，aro-疫苗菌株）为25%。（摘要截短于250字）
