Clarke R C, Gyles C L
Can J Vet Res. 1987 Jan;51(1):32-8.
The purpose of this study was to evaluate the safety and efficacy of a diaminopimelic acid mutant of Salmonella typhimurium as a vaccine for calves. Transposon techniques were used to create a stable nonreverting diaminopimelic acid mutant of a virulent S. typhimurium strain. Calves were vaccinated at weekly intervals with the diaminopimelic acid mutant given as an oral dose of 10(10) organisms, followed by two subcutaneous doses of 10(9) organisms. The calves tolerated vaccination well and the vaccine strain was eliminated from the feces within four days. Of five vaccinated calves, three survived challenge with 5 X 10(9) organisms of the parent strain whereas all five unvaccinated calves that were challenged died. The surviving calves eliminated the challenge organism from the feces within three weeks.
本研究的目的是评估鼠伤寒沙门氏菌二氨基庚二酸突变体作为犊牛疫苗的安全性和有效性。采用转座子技术构建了一株稳定的、不回复突变的强毒株鼠伤寒沙门氏菌二氨基庚二酸突变体。犊牛每周口服10(10)个菌量的二氨基庚二酸突变体进行疫苗接种，随后皮下注射两次10(9)个菌量。犊牛对疫苗接种耐受性良好，疫苗菌株在四天内从粪便中清除。在五只接种疫苗的犊牛中，三只在受到5×10(9)个亲本菌株菌量的攻击后存活，而所有五只受到攻击的未接种疫苗的犊牛均死亡。存活的犊牛在三周内从粪便中清除了攻击菌株。
