Wray C, Sojka W J, Pritchard D G, Morris J A
Dev Biol Stand. 1983;53:41-6.
A galactose epimeraseless (gal E) mutant of Salmonella typhimurium was investigated in mice, calves and poultry for its suitability as a live vaccine. In mice a very highly significant difference in the mortality rates was observed when vaccinated and non-vaccinated animals were challenged with virulent strains of S. typhimurium and S. dublin. In calves, doses of 10(6) and above of gal E mutant injected subcutaneously provided highly significant protection in terms of mortality and prevalence of symptoms when challenged orally with S. typhimurium. Renal lesions were found in a number of calves and studies were undertaken to investigate their pathogenesis. The results of these studies suggested that gal E. mutants were unlikely to produce the renal lesions. When day-old chicks were vaccinated orally with live gal E mutant of S. typhimurium and challenged orally 14 days later there was a statistically significant reduction in faecal shedding, in the salmonella carrier status at slaughter and in the numbers of salmonellae in the broiler house environment. The vaccine however, had a significant effect on the live weight gain of the birds.
对鼠伤寒沙门氏菌的一种无半乳糖差向异构酶（gal E）突变体在小鼠、犊牛和家禽中进行了研究，以考察其作为活疫苗的适用性。在小鼠中，当用鼠伤寒沙门氏菌和都柏林沙门氏菌的强毒株对接种疫苗和未接种疫苗的动物进行攻毒时，观察到死亡率存在非常显著的差异。在犊牛中，皮下注射10⁶及以上剂量的gal E突变体，在口服鼠伤寒沙门氏菌攻毒时，在死亡率和症状发生率方面提供了高度显著的保护。在一些犊牛中发现了肾脏病变，并开展了研究以调查其发病机制。这些研究结果表明，gal E突变体不太可能引发肾脏病变。当用鼠伤寒沙门氏菌的活gal E突变体对1日龄雏鸡进行口服接种，并在14天后进行口服攻毒时，粪便排菌量、屠宰时沙门氏菌携带状态以及肉鸡舍环境中的沙门氏菌数量均有统计学意义的显著减少。然而，该疫苗对家禽的体重增加有显著影响。
