Lur Gyorgy, Vinck Martin A, Tang Lan, Cardin Jessica A, Higley Michael J
Department of Neuroscience, Kavli Institute for Neuroscience, Yale School of Medicine, New Haven, CT 06520, USA; Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale School of Medicine, New Haven, CT 06520, USA.
Department of Neuroscience, Kavli Institute for Neuroscience, Yale School of Medicine, New Haven, CT 06520, USA.
Cell Rep. 2016 Mar 22;14(11):2538-45. doi: 10.1016/j.celrep.2016.02.050. Epub 2016 Mar 10.
Primary neocortical sensory areas act as central hubs, distributing afferent information to numerous cortical and subcortical structures. However, it remains unclear whether each downstream target receives a distinct version of sensory information. We used in vivo calcium imaging combined with retrograde tracing to monitor visual response properties of three distinct subpopulations of projection neurons in primary visual cortex. Although there is overlap across the groups, on average, corticotectal (CT) cells exhibit lower contrast thresholds and broader tuning for orientation and spatial frequency in comparison to corticostriatal (CS) cells, whereas corticocortical (CC) cells have intermediate properties. Noise correlational analyses support the hypothesis that CT cells integrate information across diverse layer 5 populations, whereas CS and CC cells form more selectively interconnected groups. Overall, our findings demonstrate the existence of functional subnetworks within layer 5 that may differentially route visual information to behaviorally relevant downstream targets.
初级新皮质感觉区充当着中枢枢纽，将传入信息分配到众多皮质和皮质下结构。然而，每个下游靶点是否接收到不同版本的感觉信息仍不清楚。我们使用体内钙成像结合逆行追踪技术，来监测初级视觉皮质中三个不同亚群投射神经元的视觉反应特性。尽管各组之间存在重叠，但平均而言，与皮质纹状体（CS）细胞相比，皮质顶盖（CT）细胞表现出更低的对比度阈值以及对方向和空间频率更宽的调谐，而皮质皮质（CC）细胞具有中间特性。噪声相关性分析支持这样的假设，即CT细胞整合来自不同5层群体的信息，而CS和CC细胞形成更具选择性的相互连接群体。总体而言，我们的研究结果表明5层内存在功能子网，这些子网可能将视觉信息以不同方式路由到与行为相关的下游靶点。
