Manafi M, Kneifel W
Hygiene-Institut, Universität Wien.
Zentralbl Hyg Umweltmed. 1989 Dec;189(3):225-34.
A comparison was made with different chromogenic and fluorogenic substrates, 4-methylumbelliferyl-beta-D-glucuronide (MUG), 4-nitrophenyl-beta-D-glucuronide (PNPG), 4-methylumbelliferyl-beta-D-galactopyranoside (MUGA), 2-nitrophenyl-beta-D-galactopyranoside (ONPG), 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-GAL), for the rapid and simultaneous enumeration of total coliforms and E. coli in water samples, based on 2 commercially available culture-media. The combination of the chromogenic compound X-GAL (for detecting coliforms) and of the fluorogenic compound MUG (for detecting E. coli) incorporated either into ECD agar or into lauryl sulfate broth proved to be most useful. The optimum concentration of the X-GAL/MUG supplement was (50 micrograms/ml/70 micrograms/ml) for the solid medium (EMX agar) and (60 micrograms/ml/70 micrograms/ml) for the fluid medium (LMX broth). As a result of the examination of 244 Enterobacteriaceae strains isolated from water samples and clinical material, it was shown that the use of EMX agar (LMX broth) had several advantages over conventional methods. A routine method for the analysis of water samples was proposed involving the EMX agar and the LMX broth.
基于两种市售培养基，使用不同的显色和荧光底物，即4-甲基伞形酮基-β-D-葡萄糖醛酸苷（MUG）、4-硝基苯基-β-D-葡萄糖醛酸苷（PNPG）、4-甲基伞形酮基-β-D-吡喃半乳糖苷（MUGA）、2-硝基苯基-β-D-吡喃半乳糖苷（ONPG）、5-溴-4-氯-3-吲哚基-β-D-吡喃半乳糖苷（X-GAL），对水样中的总大肠菌群和大肠杆菌进行快速同时计数。结果表明，将显色化合物X-GAL（用于检测大肠菌群）和荧光化合物MUG（用于检测大肠杆菌）添加到ECD琼脂或月桂基硫酸盐肉汤中最为有用。对于固体培养基（EMX琼脂），X-GAL/MUG补充剂的最佳浓度为（50微克/毫升/70微克/毫升），对于液体培养基（LMX肉汤），最佳浓度为（60微克/毫升/70微克/毫升）。通过对从水样和临床材料中分离出的244株肠杆菌科菌株的检测，结果表明，使用EMX琼脂（LMX肉汤）比传统方法具有多个优点。提出了一种使用EMX琼脂和LMX肉汤分析水样的常规方法。
