使用含色原底物的新型培养基对尿样中肠杆菌科细菌进行评估。
Evaluation of new medium with chromogenic substrates for members of the family Enterobacteriaceae in urine samples.
作者信息
Kodaka H, Ishikawa M, Iwata M, Kashitani F, Mizuochi S, Yamaguchi K
机构信息
Department of Microbiology, School of Medicine, Toho University, Tokyo, Japan.
出版信息
J Clin Microbiol. 1995 Jan;33(1):199-201. doi: 10.1128/jcm.33.1.199-201.1995.
Abstract
A new medium containing 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt (Glu agar) for Escherichia coli and a new medium containing 5-bromo-3-indolyl-beta-D-galactoside (Gal agar) for beta-galactosidase-positive members of the family Enterobacteriaceae were compared with MacConkey agar in a diagnostic trial with 3,562 urine specimens. The isolation rates of E. coli and beta-galactosidase-positive Enterobacteriaceae were increased 8.4 and 19.5%, respectively. The sensitivities and specificities of Glu agar and Gal agar were 98.5 and 100% and 99.2 and 99.5%, respectively.
摘要
一种含有5-溴-4-氯-3-吲哚基-β-D-葡糖醛酸环己铵盐（Glu琼脂）用于培养大肠杆菌的新型培养基，以及一种含有5-溴-3-吲哚基-β-D-半乳糖苷（Gal琼脂）用于培养肠杆菌科β-半乳糖苷酶阳性菌的新型培养基，在一项对3562份尿液标本的诊断试验中与麦康凯琼脂进行了比较。大肠杆菌和β-半乳糖苷酶阳性肠杆菌科细菌的分离率分别提高了8.4%和19.5%。Glu琼脂和Gal琼脂的敏感性和特异性分别为98.5%和100%以及99.2%和99.5%。
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