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使用含色原底物的新型培养基对尿样中肠杆菌科细菌进行评估。

Evaluation of new medium with chromogenic substrates for members of the family Enterobacteriaceae in urine samples.

作者信息

Kodaka H, Ishikawa M, Iwata M, Kashitani F, Mizuochi S, Yamaguchi K

机构信息

Department of Microbiology, School of Medicine, Toho University, Tokyo, Japan.

出版信息

J Clin Microbiol. 1995 Jan;33(1):199-201. doi: 10.1128/jcm.33.1.199-201.1995.

DOI:10.1128/jcm.33.1.199-201.1995
PMID:7699041
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC227907/
Abstract

A new medium containing 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt (Glu agar) for Escherichia coli and a new medium containing 5-bromo-3-indolyl-beta-D-galactoside (Gal agar) for beta-galactosidase-positive members of the family Enterobacteriaceae were compared with MacConkey agar in a diagnostic trial with 3,562 urine specimens. The isolation rates of E. coli and beta-galactosidase-positive Enterobacteriaceae were increased 8.4 and 19.5%, respectively. The sensitivities and specificities of Glu agar and Gal agar were 98.5 and 100% and 99.2 and 99.5%, respectively.

摘要

一种含有5-溴-4-氯-3-吲哚基-β-D-葡糖醛酸环己铵盐(Glu琼脂)用于培养大肠杆菌的新型培养基,以及一种含有5-溴-3-吲哚基-β-D-半乳糖苷(Gal琼脂)用于培养肠杆菌科β-半乳糖苷酶阳性菌的新型培养基,在一项对3562份尿液标本的诊断试验中与麦康凯琼脂进行了比较。大肠杆菌和β-半乳糖苷酶阳性肠杆菌科细菌的分离率分别提高了8.4%和19.5%。Glu琼脂和Gal琼脂的敏感性和特异性分别为98.5%和100%以及99.2%和99.5%。

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本文引用的文献

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Indoxyl-beta-D-glucuronide, a novel chromogenic reagent for the specific detection and enumeration of Escherichia coli in environmental samples.吲哚酚-β-D-葡萄糖醛酸,一种用于环境样品中大肠杆菌特异性检测和计数的新型显色试剂。
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Novel compound for identifying Escherichia coli.用于鉴定大肠杆菌的新型化合物。
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Zentralbl Hyg Umweltmed. 1989 Dec;189(3):225-34.
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J Clin Microbiol. 1989 Apr;27(4):778-9. doi: 10.1128/jcm.27.4.778-779.1989.
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