Božič Abram Sabina, Aupič Jana, Dražić Goran, Gradišar Helena, Jerala Roman
Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia; Graduate School of Biomedicine, University of Ljubljana, Ljubljana 1000, Slovenia.
Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia; Doctoral Programme in Chemical Sciences, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana 1000, Slovenia.
Biochem Biophys Res Commun. 2016 Apr 8;472(3):566-71. doi: 10.1016/j.bbrc.2016.03.042. Epub 2016 Mar 10.
Biopolymers with defined sequence patterns offer an attractive alternative for the formation of silver nanoparticle (AgNP). A set of coiled-coil dimer forming peptides was tested for their AgNP formation ability. Seventeen of those peptides mediated the formation of AgNPs in aqueous solution at neutral pH, while the formation of a coiled-coil dimer inhibited the nanoparticle generation. A QSAR regression model on the relationship between sequence and function suggests that in this peptide type the patterns KXQQ and KXEE are favorable, whereas Ala residues appear to have an inhibitory effect. UV-VIS spectra of the obtained nanoparticles gave a peak at around 420 nm, typical for AgNPs in the size range around 40 nm, which was confirmed by dynamic light scattering and transmission electron microscopy. Peptide-induced AgNPs exhibited good antibacterial activity, even after a 15 min contact time, while they had low toxicity to human cells at the same concentrations. These results show that our designed peptides generate AgNPs with antibacterial activity at mild conditions and might be used for antibacterial coatings.
具有特定序列模式的生物聚合物为银纳米颗粒（AgNP）的形成提供了一种有吸引力的替代方案。测试了一组形成卷曲螺旋二聚体的肽的AgNP形成能力。其中17种肽在中性pH的水溶液中介导了AgNP的形成，而卷曲螺旋二聚体的形成则抑制了纳米颗粒的产生。关于序列与功能关系的定量构效关系（QSAR）回归模型表明，在这种肽类型中，模式KXQQ和KXEE是有利的，而丙氨酸残基似乎具有抑制作用。所获得的纳米颗粒的紫外可见光谱在约420nm处有一个峰值，这对于尺寸在约40nm范围内的AgNP来说是典型的，动态光散射和透射电子显微镜证实了这一点。肽诱导的AgNP即使在接触15分钟后仍表现出良好的抗菌活性，而在相同浓度下它们对人类细胞的毒性较低。这些结果表明，我们设计的肽在温和条件下产生具有抗菌活性的AgNP，可能用于抗菌涂层。
