Chu Chunjun, Ren Huiling, Xu Naiyu, Xia Long, Chen Daofeng, Zhang Jian
College of Pharmaceutical Science, Soochow University, Suzhou 215123, PR China.
Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, PR China.
J Ethnopharmacol. 2016 Jun 5;185:263-71. doi: 10.1016/j.jep.2016.03.022. Epub 2016 Mar 11.
Eupatorium lindleyanum DC. is widely used for its efficiency in treating cough, tracheitis and tonsillitis. Acute lung injury (ALI) induced by lipopolysaccharide (LPS) in mice was used to investigate therapeutic effects and possible mechanism of the sesquiterpenes fraction of E. lindleyanum DC. (EUP-SQT).
Mice were orally administrated with EUP-SQT (15, 30 and 60mg/kg) per day for 7 days consecutively before LPS challenge. The lung specimens and bronchoalveolar lavage fluid (BALF) were harvested for histopathological examinations and biochemical analysis at 6h and 24h after LPS challenge. The level of complement 3 (C3) and complement 3c (C3c) in serum was quantified by a sandwich ELISA kit.
Pretreatment with EUP-SQT could significantly decrease lung wet-to-dry weight (W/D) ratio, nitric oxide (NO) and protein concentration in BALF, which was exhibited together with the lowered myeloperoxidase (MPO) activity, the increased superoxide dismutase (SOD) activity and down-regulation the level of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) in ALI model. Additionally, EUP-SQT attenuated lung histopathological changes and significantly reduced complement deposition with decreasing the level of C3 and C3c in serum.
These results showed that EUP-SQT significantly attenuated LPS-induced ALI via reducing productions of pro-inflammatory mediators and decreasing the level of complement, indicating it as a potential therapeutic agent for ALI.
林泽兰被广泛用于治疗咳嗽、气管炎和扁桃体炎。采用脂多糖（LPS）诱导的小鼠急性肺损伤（ALI）模型，研究林泽兰倍半萜组分（EUP-SQT）的治疗作用及其可能机制。
在LPS攻击前，小鼠连续7天每天口服EUP-SQT（15、30和60mg/kg）。在LPS攻击后6小时和24小时，采集肺组织标本和支气管肺泡灌洗液（BALF）进行组织病理学检查和生化分析。采用夹心ELISA试剂盒定量检测血清中补体3（C3）和补体3c（C3c）水平。
EUP-SQT预处理可显著降低ALI模型小鼠肺组织湿干重（W/D）比值、BALF中一氧化氮（NO）和蛋白质浓度，同时降低髓过氧化物酶（MPO）活性，增加超氧化物歧化酶（SOD）活性，并下调肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）水平。此外，EUP-SQT减轻了肺组织病理学变化，显著减少补体沉积，降低血清中C3和C3c水平。
这些结果表明，EUP-SQT通过减少促炎介质的产生和降低补体水平，显著减轻LPS诱导的ALI，提示其可能是一种治疗ALI的潜在药物。
