College of Pharmaceutical Science, Soochow University, Suzhou 215123, People's Republic of China.
College of Pharmaceutical Science, Soochow University, Suzhou 215123, People's Republic of China; Pharmacy Department of BOE Hefei Digital Hospital, Hefei 230000, People's Republic of China.
J Pharmacol Sci. 2019 Jul;140(3):228-235. doi: 10.1016/j.jphs.2019.07.004. Epub 2019 Jul 18.
Acute lung injury (ALI) results from various factors including uncontrolled pulmonary inflammation, oxidative damage and the over-activated complement with high mortality rates. Jaceosidin was a flavonoid compound with significant anti-complement activity. We aimed to investigate the therapeutic effects of Jaceosidin on ALI induced by lipopolysaccharide (LPS). Mice were orally administrated with Jaceosidin (15, 30 and 60 mg/kg) after LPS challenge. 24 h after LPS challenge, Jaceosidin could significantly decrease the lung wet-to-dry weight (W/D) ratio and the protein concentration in bronchoalveolar lavage fluid (BALF). Jaceosidin could down-regulate the levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β), together with up-regulation the levels of interleukin-4 (IL-4) and interleukin-10 (IL-10) in BALF. Jaceosidin could significantly decrease the levels of myeloperoxidase (MPO), cyclooxygenase-2 (COX-2) and nuclear factor-κB (NF-κB), COX-2 mRNA and NF-κB p65 mRNA together with increasing the activity of catalase (CAT). Additionally, Jaceosidin attenuated lung histopathological changes, inhibited the expressions of COX-2 and NF-κB p65 and reduced complement deposition with decreasing the levels of complement 3 (C3) and complement 3c (C3c) in serum. These data suggest that Jaceocidin may dampen the inflammatory response and decrease the levels of complement together with the antioxidant activity following LPS-induced ALI.
急性肺损伤（ALI）是由多种因素引起的，包括失控的肺部炎症、氧化损伤和过度激活的补体，其死亡率很高。乔松素是一种具有显著抗补体活性的黄酮类化合物。我们旨在研究乔松素对脂多糖（LPS）诱导的 ALI 的治疗作用。LPS 攻击后，小鼠经口给予乔松素（15、30 和 60mg/kg）。LPS 攻击后 24 小时，乔松素可显著降低肺湿重/干重（W/D）比和支气管肺泡灌洗液（BALF）中的蛋白浓度。乔松素可下调 BALF 中肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）的水平，同时上调白细胞介素-4（IL-4）和白细胞介素-10（IL-10）的水平。乔松素可显著降低髓过氧化物酶（MPO）、环加氧酶-2（COX-2）和核因子-κB（NF-κB）、COX-2 mRNA 和 NF-κB p65 mRNA 的水平，同时增加过氧化氢酶（CAT）的活性。此外，乔松素减轻了肺组织病理学变化，抑制了 COX-2 和 NF-κB p65 的表达，并减少了补体沉积，同时降低了血清中补体 3（C3）和补体 3c（C3c）的水平。这些数据表明，乔松素可能通过抑制炎症反应和降低补体水平以及发挥抗氧化活性，减轻 LPS 诱导的 ALI。
