Cozza Natascia, Bonani Walter, Motta Antonella, Migliaresi Claudio
Department of Industrial Engineering and BIOtech Research Center, University of Trento, Trento, Italy.
Department of Industrial Engineering and BIOtech Research Center, University of Trento, Trento, Italy; European Institute of Excellence on Tissue Engineering and Regenerative Medicine and INSTM Research Center, Trento Research Unit, Trento, Italy.
Int J Biol Macromol. 2016 Jun;87:504-13. doi: 10.1016/j.ijbiomac.2016.03.013. Epub 2016 Mar 10.
Acid-Solubilized Collagen (ASC) and Pepsin-Solubilized Collagen (PSC) were extracted from the mantle of the common European squid, and were comparatively characterized. ASC and PSC were isolated with an extraction yield of 5.1 and 24.2% (on dry weight basis), respectively. SDS-PAGE showed that the ASC was mostly comprised of α1- and α2-chains; while the PSC presented relevant β- and γ-components. GPC analysis confirmed that both the ASC and the PSC consisted of fractions characterized by different molecular weight. Thermal denaturation behavior of ASC and PSC were followed by calorimetric and rheological analyses; denaturation temperature was estimated to be 22°C for ASC and 21°C for PSC. Amino acid composition and solubility of collagen were also investigated. Finally, the cytotoxicity of the isolated collagen was evaluated in vitro and no cytotoxic activity caused by the collagen extracts was observed. This study demonstrated that squid mantle has potential as an alternative source of collagen-derived materials.
从欧洲普通鱿鱼的外套膜中提取了酸溶性胶原蛋白（ASC）和胃蛋白酶溶性胶原蛋白（PSC），并对其进行了比较表征。ASC和PSC的提取率分别为5.1%和24.2%（以干重计）。SDS-PAGE显示ASC主要由α1链和α2链组成；而PSC呈现出相关的β和γ组分。凝胶渗透色谱分析证实ASC和PSC均由具有不同分子量特征的组分组成。通过量热分析和流变分析研究了ASC和PSC的热变性行为；ASC的变性温度估计为22℃，PSC为21℃。还研究了胶原蛋白的氨基酸组成和溶解性。最后，在体外评估了分离出的胶原蛋白的细胞毒性，未观察到胶原蛋白提取物引起的细胞毒性活性。这项研究表明，鱿鱼外套膜有潜力作为胶原蛋白衍生材料的替代来源。
