State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266580, China.
Yantai Institute of Coastal Zone, Chinese Academy of Sciences, Yantai, 264003, China.
Mar Drugs. 2020 Mar 25;18(4):178. doi: 10.3390/md18040178.
Collagen plays an important role in the formation of extracellular matrix (ECM) and development/migration of cells and tissues. Here we report the preparation of collagen and collagen hydrogel from the skin of tilapia and an evaluation of their potential as a wound dressing for the treatment of refractory wounds. The acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted and characterized using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), differential scanning calorimetry (DSC), circular dichroism (CD) and Fourier transform infrared spectroscopy (FTIR) analysis. Both ASC and PSC belong to type I collagen and have a complete triple helix structure, but PSC shows lower molecular weight and thermal stability, and has the inherent low antigenicity. Therefore, PSC was selected to prepare biomedical hydrogels using its self-aggregating properties. Rheological characterization showed that the mechanical strength of the hydrogels increased as the PSC content increased. Scanning electron microscope (SEM) analysis indicated that hydrogels could form a regular network structure at a suitable PSC content. Cytotoxicity experiments confirmed that hydrogels with different PSC content showed no significant toxicity to fibroblasts. Skin repair experiments and pathological analysis showed that the collagen hydrogels wound dressing could significantly accelerate the healing of deep second-degree burn wounds and the generation of new skin appendages, which can be used for treatment of various refractory wounds.
胶原蛋白在细胞和组织的发育/迁移以及细胞外基质（ECM）的形成中起着重要作用。在这里，我们报告了从罗非鱼皮中制备胶原蛋白和胶原蛋白水凝胶，并评估了它们作为治疗难治性伤口的伤口敷料的潜力。使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）、差示扫描量热法（DSC）、圆二色性（CD）和傅里叶变换红外光谱（FTIR）分析对酸溶性胶原蛋白（ASC）和胃蛋白酶溶性胶原蛋白（PSC）进行了提取和表征。ASC 和 PSC 均属于 I 型胶原蛋白，具有完整的三螺旋结构，但 PSC 具有较低的分子量和热稳定性，并且具有固有的低抗原性。因此，选择 PSC 利用其自聚集特性来制备生物医学水凝胶。流变学特性表明，水凝胶的机械强度随 PSC 含量的增加而增加。扫描电子显微镜（SEM）分析表明，水凝胶在合适的 PSC 含量下可以形成规则的网络结构。细胞毒性实验证实，具有不同 PSC 含量的水凝胶对成纤维细胞没有明显的毒性。皮肤修复实验和病理分析表明，胶原蛋白水凝胶伤口敷料能显著促进深二度烧伤创面的愈合和新皮肤附属物的生成，可用于治疗各种难治性创面。
