[生理妊娠时羊水基质金属蛋白酶-1、-2、-9及基质金属蛋白酶组织抑制剂-1浓度的参考范围]

[Reference ranges of matrix metalloproteinase-1, -2, -9 and tissue inhibitor of matrix metalloproteinases-1 concentrations in amniotic fluid in physiological pregnancy].

作者信息

Korenovsky Yu V, Remneva O V

机构信息

Altai State Medical University, Barnaul, Altaiskii Region, Russia.

出版信息

Biomed Khim. 2016 Jan-Feb;62(1):96-8. doi: 10.18097/PBMC20166201096.

DOI:10.18097/PBMC20166201096

PMID:26973196

Abstract

The aim of this study was to determine reference values of matrix metalloproteinase-1 (MMP-1), MMP-2, MMP-9 and tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) in the amniotic fluid at the first stage of labor in physiological pregnancy. 89 women at the first stage of term labor have been examined. Samples of amniotic fluid were taken at the first period of labor by vaginal amniotomy. Concentrations ofMMP-1, MMP-2, MMP-9, and TIMP-1 were investigated in amniotic fluid by ELISA kits. We have determined normal concentration ranges for MMP-1, MMP-2, MMP-9, TIMP-1, and ratios of concentrations of MMPs and TIMP-1 (MMP-1/TIMP-1, MMP-2/TIMP-1, MMP-9/TIMP-1) in the amniotic fluid at the first period of labor in physiological pregnancy. These included: MMP-1--5.1-16.8 pg/mg of protein, MMP-2--238.3-374.1 pg/mg of protein, MMP-9--66.1-113.3 pg/mg of protein, TIMP-1--4.7-13.6 pg/mg of protein, ratio of MMP-1/TIMP-1--0.1-2.2, ratio of MMP-2/TIMP-1--19.9-55.7, ratio of MMP-9/TIMP-1--4.2-17.2.

摘要

本研究的目的是确定生理妊娠第一产程时羊水中基质金属蛋白酶-1（MMP-1）、MMP-2、MMP-9和基质金属蛋白酶组织抑制剂-1（TIMP-1）的参考值。对89名足月第一产程的妇女进行了检查。在第一产程初期通过阴道羊膜穿刺术采集羊水样本。采用酶联免疫吸附测定试剂盒检测羊水中MMP-1、MMP-2、MMP-9和TIMP-1的浓度。我们确定了生理妊娠第一产程时羊水中MMP-1、MMP-2、MMP-9、TIMP-1的正常浓度范围以及MMP与TIMP-1浓度之比（MMP-1/TIMP-1、MMP-2/TIMP-1、MMP-9/TIMP-1）。这些范围包括：MMP-1——5.1 - 16.8 pg/mg蛋白质，MMP-2——238.3 - 374.1 pg/mg蛋白质，MMP-9——66.1 - 113.3 pg/mg蛋白质，TIMP-1——4.7 - 13.6 pg/mg蛋白质，MMP-1/TIMP-1之比——0.1 - 2.2，MMP-2/TIMP-1之比——19.9 - 55.7，MMP-9/TIMP-1之比——4.2 - 17.2。