Yagi Hideki, Sumino Hiroyuki, Yoshida Kensuke, Aoki Tomoyuki, Tsunekawa Katsuhiko, Araki Osamu, Kimura Takao, Nara Makoto, Nakajima Katsuyuki, Murakami Masami
Department of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine.
Int Heart J. 2016;57(2):220-5. doi: 10.1536/ihj.15-389. Epub 2016 Mar 11.
Oxidative stress is a crucial factor in the pathogenesis and development of cardiovascular disease. Recently, simplified methods for the detection of reactive oxygen species (ROS) using the derivatives of reactive oxygen metabolites (d-ROMs) test as an index of ROS products and the biological antioxidant potential (BAP) test as an index of antioxidant potential have been utilized. These methods are easy to perform, quick, inexpensive since they use small equipment, and provide reliable results compared with established oxidative stress and antioxidant markers. Because oxidative stress has been shown to represent the balance of production of ROS and antioxidant capacity, it is more appropriate to evaluate ROS and antioxidant capacity simultaneously. However, no study has examined the associations among d-ROMs, BAP values, and carotid artery intima-media thickness (IMT) concurrently. Therefore, we studied the associations among d-ROMs, BAP values, and the carotid artery IMT. Carotid artery IMT, blood pressure (BP), fasting circulating d-ROMs, BAP, glucose metabolism, lipid, and C-reactive protein levels were measured in 95 subjects (age: 49.5 ± 13.8 years; men: 41; women: 54), including 42 healthy subjects and 53 patients with hypertension, dyslipidemia, and diabetes mellitus who were not on medication. The results of multiple regression analysis revealed that dependent carotid artery IMT determinants remained significantly associated with age, systolic BP, total cholesterol, and BAP, whereas dependent BAP determinants remained significantly associated with body mass index and carotid artery IMT. BAP was strongly correlated with carotid artery IMT in our cohort. Our results suggest that BAP may be a useful risk marker for carotid atherosclerosis.
氧化应激是心血管疾病发病机制和发展过程中的一个关键因素。最近，已经采用了一些简化方法来检测活性氧（ROS），这些方法使用活性氧代谢产物衍生物（d-ROMs）测试作为ROS产物的指标，以及生物抗氧化潜力（BAP）测试作为抗氧化潜力的指标。这些方法易于操作、快速、成本低廉，因为它们使用的设备较小，并且与已有的氧化应激和抗氧化标志物相比，能提供可靠的结果。由于氧化应激已被证明代表ROS产生和抗氧化能力的平衡，同时评估ROS和抗氧化能力更为合适。然而，尚无研究同时考察d-ROMs、BAP值与颈动脉内膜中层厚度（IMT）之间的关联。因此，我们研究了d-ROMs、BAP值与颈动脉IMT之间的关联。对95名受试者（年龄：49.5±13.8岁；男性：41名；女性：54名）进行了颈动脉IMT、血压（BP）、空腹循环d-ROMs、BAP、糖代谢、血脂和C反应蛋白水平的测量，其中包括42名健康受试者和53名未服用药物的高血压、血脂异常和糖尿病患者。多元回归分析结果显示，颈动脉IMT的相关决定因素仍然与年龄、收缩压、总胆固醇和BAP显著相关，而BAP的相关决定因素仍然与体重指数和颈动脉IMT显著相关。在我们的队列中，BAP与颈动脉IMT密切相关。我们的结果表明，BAP可能是颈动脉粥样硬化的一个有用的风险标志物。
