Hudson-Lamb Gavin C, Schoeman Johan P, Hooijberg Emma H, Heinrich Sonja K, Tordiffe Adrian S W
Department of Companion Animal Clinical Studies, University of Pretoria.
J S Afr Vet Assoc. 2016 Feb 26;87(1):1316. doi: 10.4102/jsava.v87i1.1316.
Published haematologic and serum biochemistry reference intervals are very scarce for captive cheetahs and even more for free-ranging cheetahs. The current study was performed to establish reference intervals for selected serum biochemistry analytes in cheetahs. Baseline serum biochemistry analytes were analysed from 66 healthy Namibian cheetahs. Samples were collected from 30 captive cheetahs at the AfriCat Foundation and 36 free-ranging cheetahs from central Namibia. The effects of captivity-status, age, sex and haemolysis score on the tested serum analytes were investigated. The biochemistry analytes that were measured were sodium, potassium, magnesium, chloride, urea and creatinine. The 90% confidence interval of the reference limits was obtained using the non-parametric bootstrap method. Reference intervals were preferentially determined by the non-parametric method and were as follows: sodium (128 mmol/L - 166 mmol/L), potassium (3.9 mmol/L - 5.2 mmol/L), magnesium (0.8 mmol/L - 1.2 mmol/L), chloride (97 mmol/L - 130 mmol/L), urea (8.2 mmol/L - 25.1 mmol/L) and creatinine (88 µmol/L - 288 µmol/L). Reference intervals from the current study were compared with International Species Information System values for cheetahs and found to be narrower. Moreover, age, sex and haemolysis score had no significant effect on the serum analytes in this study. Separate reference intervals for captive and free-ranging cheetahs were also determined. Captive cheetahs had higher urea values, most likely due to dietary factors. This study is the first to establish reference intervals for serum biochemistry analytes in cheetahs according to international guidelines. These results can be used for future health and disease assessments in both captive and free-ranging cheetahs.
已发表的圈养猎豹血液学和血清生化参考区间非常稀少，对于野生猎豹更是如此。本研究旨在建立猎豹特定血清生化分析物的参考区间。对66只健康的纳米比亚猎豹进行了基线血清生化分析物检测。样本采集自阿弗里卡特基金会的30只圈养猎豹以及纳米比亚中部的36只野生猎豹。研究了圈养状态、年龄、性别和溶血评分对所测血清分析物的影响。所测的生化分析物包括钠、钾、镁、氯、尿素和肌酐。参考限值的90%置信区间采用非参数自助法获得。参考区间优先采用非参数方法确定，结果如下：钠（128毫摩尔/升 - 166毫摩尔/升），钾（3.9毫摩尔/升 - 5.2毫摩尔/升），镁（0.8毫摩尔/升 - 1.2毫摩尔/升），氯（97毫摩尔/升 - 130毫摩尔/升），尿素（8.2毫摩尔/升 - 25.1毫摩尔/升）和肌酐（88微摩尔/升 - 288微摩尔/升）。将本研究的参考区间与国际物种信息系统中猎豹的值进行比较，发现更窄。此外，在本研究中年龄、性别和溶血评分对血清分析物无显著影响。还确定了圈养和野生猎豹各自的参考区间。圈养猎豹的尿素值较高，很可能是由于饮食因素。本研究是首次根据国际指南建立猎豹血清生化分析物的参考区间。这些结果可用于未来圈养和野生猎豹的健康与疾病评估。
