Subochev Pavel
Opt Lett. 2016 Mar 1;41(5):1006-9. doi: 10.1364/OL.41.001006.
The idea of a method of cost-effective upgrades from an acoustic resolution photoacoustic microscope to a triple-modality imaging system is validated using phantoms. The newly developed experimental setup is based on a diode pumped solid state laser coupled to a fiber bundle with a spherically focused polyvinylidene fluoride detector integrated into the center of a ring shaped optical illuminator. Each laser pulse illuminating the sample performs two functions. While the photons absorbed by the sample provide a measurable optoacoustic (OA) signal, the photons absorbed by the detector provide the measurable diffuse reflectometry (DR) signal from the sample and the probing ultrasonic (US) pulse. At a 3 mm imaging depth, the axial resolution of the OA/US modalities is 38 μm/26 μm, while the lateral resolution of the DR/OA/US modalities is 3.5 mm/50 μm/35 μm. The maximum acquisition rate of the trimodal DR/OA/US A-scans is 2 kHz.
使用体模验证了一种从声学分辨率光声显微镜经济高效升级到三模态成像系统的方法的想法。新开发的实验装置基于一个二极管泵浦固态激光器，该激光器与一个光纤束耦合，在环形光学照明器的中心集成了一个球形聚焦的聚偏二氟乙烯探测器。照射样品的每个激光脉冲执行两种功能。被样品吸收的光子提供可测量的光声（OA）信号，而被探测器吸收的光子提供来自样品的可测量的漫反射测量（DR）信号和探测超声（US）脉冲。在3毫米的成像深度处，OA/US模态的轴向分辨率为38微米/26微米，而DR/OA/US模态的横向分辨率为3.5毫米/50微米/35微米。三模态DR/OA/US A扫描的最大采集速率为2千赫兹。
