Benito-Alifonso David, Tremell Shirley, Sadler Joanna C, Berry Monica, Galan M Carmen
School of Chemistry, University of Bristol, Cantock's Close, BS8 1TS, UK.
Chem Commun (Camb). 2016 Apr 7;52(27):4906-9. doi: 10.1039/c5cc10040b. Epub 2016 Mar 14.
Selective, bioorthogonal and fast labeling of glycoconjugates in living cells is a major challenge for synthetic and cellular biology. Here we report the use imidazolium tagged-mannosamine derivative (ITag-Man) for the non-covalent, rapid and site-specific labeling of sialic acid containing glycoproteins using commercial N-nitrilotriacetate fluorescent reagents in a range of cell lines.
