Horner Patrick J, Wills Gillian S, Righarts Antoinette, Vieira Sueli, Kounali Daphne, Samuel Dhanraj, Winston Alan, Muir David, Dickson Nigel P, McClure Myra O
Jefferiss Trust Laboratories, Wright-Fleming Institute, Imperial College London, London, United Kingdom.
School of Social and Community Medicine, University of Bristol, Bristol, United Kingdom.
PLoS One. 2016 Mar 14;11(3):e0151497. doi: 10.1371/journal.pone.0151497. eCollection 2016.
Chlamydia trachomatis (Ct) serological studies in populations could help monitor changes in lifetime cumulative risk of infection. We developed a double-antigen sandwich ELISA based on the Ct-specific Pgp3 antigen, then tested blind stored sera from over 800 participants in a New Zealand birth cohort from Dunedin at ages 26, 32 and 38. The double-antigen sandwich ELISA was more sensitive than our previously characterised indirect Pgp3 ELISA. Pgp3 antibody was detected more often in women compared to men and correlated with increasing numbers of sexual partners, self-reported Ct, and younger age at sexual debut in both women and men. At age 26, 24.1% (99/411) of women were Pgp3 seropositive, as were 79.5% (35/44) of those reporting Ct infection; Pgp3 antibody persisted to age 38 in 96.5% (83/86). In men at age 26, the figures were 10.7% (47/442) and 25.0% (6/24), respectively, with high (83.9%) antibody persistence to age 38. At age 38, among those Pgp3 seropositive, 63.3% of women and 83.1% of men had not reported Ct infection. Thus, Ct-specific Pgp3 antibody was detected in most women reporting Ct infection and correlated with risk of infection in those who did not, with most infections remaining undetected. As this antibody persisted for at least twelve years in 96% of these women, serology could be used to evaluate Ct prevention programmes among women.
对人群进行沙眼衣原体（Ct）血清学研究有助于监测一生中累积感染风险的变化。我们基于Ct特异性Pgp3抗原开发了一种双抗原夹心ELISA，然后对来自达尼丁的一个新西兰出生队列中800多名参与者在26岁、32岁和38岁时的盲存血清进行了检测。双抗原夹心ELISA比我们之前鉴定的间接Pgp3 ELISA更敏感。与男性相比，女性中检测到Pgp3抗体的情况更常见，并且与性伴侣数量增加、自我报告的Ct感染以及男女首次性行为时的年龄较小相关。在26岁时，24.1%（99/411）的女性Pgp3血清学呈阳性，报告有Ct感染的女性中这一比例为79.5%（35/44）；96.5%（83/86）的女性Pgp3抗体持续到38岁。在26岁的男性中，相应的数字分别为10.7%（47/442）和25.0%（6/24），到38岁时抗体持续率较高（83.9%）。在38岁时，在那些Pgp3血清学呈阳性的人中，63.3%的女性和83.1%的男性未报告有Ct感染。因此，在大多数报告有Ct感染的女性中检测到了Ct特异性Pgp3抗体，并且与未感染女性的感染风险相关，大多数感染仍未被发现。由于96%的这些女性中这种抗体至少持续了12年，血清学可用于评估女性中的Ct预防项目。
