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不同氮素形态及比例对桔梗生长和活性成分含量的影响

[Effect of different nitrogen forms and ratio on growth and active ingredient content of Platycodon grandiflorum].

作者信息

Duan Yun-jing, Wang Kang-cai, Niu Ling-hui, Li Ke, Su Yun-yun

出版信息

Zhongguo Zhong Yao Za Zhi. 2015 Oct;40(19):3754-9.

Abstract

To providing evidence about nitrogen adequate application of Platycodon grandiflorum, the pot culture experiment was conducted to study the effect of nitrogen on the growth, physiological metabolism and the quality of P. grandiflorum. The activity of NR, GS and SOD, POD and CAT were determined. And the nitrate and ammonium nitrogen content, photosynthetic characteristics, active components of P. grandiflorum were determined. The results showed that the nitrate nitrogen content and P. biomass reached its maximum value, when NH4(+)-N/NO3(-) -N was 0: 100, the activity of NR. The activity of GS was the highest at the NH4(+) -N/NO3(-) -N ratio of 25:75 and ammonium nitrogen content was the highest at 75:25. The activity of SOD decreased and then increased with the increasing of NO3(-) -N. At the NH4(+) -N/NO3(-) -N ratio of 25: 75, the activity of CAT had its maximum value and the content of MDA had the minimum value. At the same time, the content of platycodon D was the highest at this treatment. The studies had shown that different nitrogen forms and ratio had a significant effect on the characteristics of photosynthetic physiology, nitrogen metabolism and resistance adjustment, growth and the quality of P. grandiflorum. The NH4(+) -N/NO3(-) -N ratio of 25: 75 was a suitable ratio of nitrogen forms for the growth of P. Grandiflorum and accumulating the content of platycodon D.

摘要

为了提供桔梗氮素适宜施用的依据,通过盆栽试验研究了氮素对桔梗生长、生理代谢及品质的影响。测定了硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性,以及桔梗的硝态氮和铵态氮含量、光合特性、活性成分。结果表明,当NH4(+)-N/NO3(-)-N为0:100时,硝态氮含量和桔梗生物量达到最大值,NR活性也最高。GS活性在NH4(+)-N/NO3(-)-N比例为25:75时最高,铵态氮含量在75:25时最高。SOD活性随NO3(-)-N含量增加先降低后升高。在NH4(+)-N/NO3(-)-N比例为25:75时,CAT活性达到最大值,丙二醛(MDA)含量达到最小值。同时,该处理下桔梗皂苷D含量最高。研究表明,不同氮素形态及比例对桔梗光合生理特性、氮代谢、抗性调节、生长及品质有显著影响。NH4(+)-N/NO3(-)-N比例为25:75是桔梗生长及积累桔梗皂苷D含量适宜的氮素形态比例。

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