Zhang Weidong, Cuperus Tryntsje, van Dijk Albert, Skjødt Karsten, Hansen Søren, Haagsman Henk P, Veldhuizen Edwin J A
Department of Infectious Diseases and Immunology, Division of Molecular Host Defence, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.
Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, DK-5000, Odense, Denmark.
Dev Comp Immunol. 2016 Aug;61:80-7. doi: 10.1016/j.dci.2016.03.010. Epub 2016 Mar 11.
Surfactant Protein A (SP-A) is a collagenous C-type lectin (collectin) that plays an important role in the early stage of the host immune response. In chicken, SP-A (cSP-A) is expressed as a 26 kDa glycosylated protein in the lung. Using immunohistochemistry, cSP-A protein was detected mainly in the lung lining fluid covering the parabronchial epithelia. Specific cSP-A producing epithelial cells, resembling mammalian type II cells, were identified in the parabronchi. Gene expression of cSP-A markedly increased from embryonic day 14 onwards until the time of hatch, comparable to the SP-A homologue chicken lung lectin, while mannan binding lectin and collectins CL-L1 and CL-K1 only showed slightly changed expression during development. cSP-A protein could be detected as early as ED 18 in lung tissue using Western blotting, and expression increased steadily until day 28 post-hatch. Our observations are a first step towards understanding the role of this protein in vivo.
表面活性蛋白A（SP-A）是一种胶原性C型凝集素（集素），在宿主免疫反应早期发挥重要作用。在鸡中，SP-A（cSP-A）在肺中表达为一种26 kDa的糖基化蛋白。利用免疫组织化学方法，在覆盖副支气管上皮的肺衬液中主要检测到了cSP-A蛋白。在副支气管中鉴定出了产生cSP-A的特异性上皮细胞，类似于哺乳动物的II型细胞。cSP-A的基因表达从胚胎第14天起显著增加，直至孵化时，这与SP-A同源物鸡肺凝集素的情况相当，而甘露聚糖结合凝集素以及集素CL-L1和CL-K1在发育过程中仅表现出轻微的表达变化。利用蛋白质印迹法，早在胚胎第18天就能在肺组织中检测到cSP-A蛋白，其表达在孵化后第28天前稳步增加。我们的观察结果是朝着理解这种蛋白在体内的作用迈出的第一步。
